CD59 anticorps (PE)

N° du produit ABIN1112195

Détail du produit anti-CD59 anticorps (PE)

Antigène: CD59

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD59 est conjugé à/à la PE

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Attributs du produit: Mouse Monoclonal Anti-Human CD59 PE is recommended for use in flow cytometry for enumerates CD59+ lymphocytes in human peripheral blood by flow cytometric methods.

Clone: VJ1-12-2

Isotype: IgG2a

Information d'application

Indications d'application: It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.

Commentaires:

R-Phycoerythrin (Europa Bioproducts, Ely, Cambridge).

Prélèvement de l'échantillon: 1. Transfer 100 µl of anticoagulated (EDTA) blood to a 12 x 75 mm polystyrene test tube (10 P6 P cells). 2. Add 1,5 ml of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 3. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 4. Add 2 ml 0.01 mol/l PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 5. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 6. Add 20 µl of CD59 PE and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 7. The recommended negative control is a non-reactive PE-conjugated antibody of the same isotype. 8. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 9. Add 2 ml 0.01 mol/l PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 10. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 11. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 ml PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 12. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.

Agent conservateur: Sodium azide

Précaution d'utilisation: 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.

Stock: 4 °C

Références pour anticorps anti-CD59 (PE) (ABIN1112195)

Hernández-Campo, Martín-Ayuso, Almeida, López, Orfao: "Comparative analysis of different flow cytometry-based immunophenotypic methods for the analysis of CD59 and CD55 expression on major peripheral blood cell subsets." dans: Cytometry, Vol. 50, Issue 3, pp. 191-201, (2002) (PubMed).

Détails sur CD59

Antigène: CD59

Autre désignation: CD59 (CD59 Produits)

Synonymes: anticorps 16.3A5, anticorps 1F5, anticorps EJ16, anticorps EJ30, anticorps EL32, anticorps G344, anticorps HRF-20, anticorps HRF20, anticorps MAC-IP, anticorps MACIF, anticorps MEM43, anticorps MIC11, anticorps MIN1, anticorps MIN2, anticorps MIN3, anticorps MIRL, anticorps MSK21, anticorps p18-20, anticorps Cd59a, anticorps Cd59b, anticorps MACIP, anticorps CD59 molecule (CD59 blood group), anticorps CD59 molecule, anticorps CD59, anticorps Cd59

Sujet: The antibody reacts with a 19kDa glycosylphosphatidylinositol (GPI) linked glycoprotein expressed on haematopoietic and non-haematopoietic cells. CD59 is expressed, like CD55, in erythrocytes. Recognizes a carbohydrate antigen present on a subset of peripheral blood mononuclear cells involved in the natural killer activity, which is not MHC restricted. The antigen is not present on B cells, monocytes, red blood cells or platelets but is expressed on cells in neuroectodermal tissue. It reacts with 20% of normal human peripheral blood mononuclear cells.

Pathways: Système du Complément