S100A9 anticorps

N° du produit ABIN111886

Détail du produit anti-S100A9 anticorps

Antigène: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp S100A9 est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: Human MRP14, Granulocytes, stimulated Monocytes and Macrophages: This clone identifies the Ca2+-binding 14 kDa subunit of the inflammatory L-1 protein complex, also called S100A9 or Calgranulin B. It is useful for the characterization of circulating granulocytes or inflammatory infiltrates of the myelo-monocytic lineage which express MRP14 differently depending on the inflammatory status of the disease. Antigen Distribution Isolated Cells: The antigen is found in granulocytes and monocytes. It is absent from all other blood cells. In cultured monocytes, maximum MRP14 expression is found after 3-4 days. Myeloid leukaemic cells have been found to be positive as well. Tissue Sections: MRP-14 is found in a distinct subpopulation of inflammatory perivascular infiltrates of the myelo-monocytic lineage. Macrophages synthesise MRP-14 increasingly during the early stages of inflammation. A high MRP-14 (and low MRP-8) expression by macrophages was reported in granulomatous diseases such as tuberculosis and sarcoidis. In non-granulomatous chronic inflammatory diseases like chronic rheumatoid arthritis, MRP8 and MRP14 positive cells consist of different subpopulations. During early inflammation endothelial cells are also positive with MRP8/14 determined by antibody 27E10 (product Cat.-No BM4025).Antigen Distribution

Réactivité croisée (Details): Species reactivity (tested):Human. The antibody reacts with Bovine spleen and not with Swine spleen.

Purification: Affinity Chromatography

Immunogène: Cultured Human monocytes. Remarks: The antigen is MRP14, the epitope is suspected in the central region of the peptide.

Clone: S36-48

Isotype: IgG1

Information d'application

Indications d'application: ELISA. Immunohistochemistry on Frozen Sections: 0.25 μg/mL (1/800)Immunohistochemistry on Paraffin Sections: 1 μg/mL (1/200). Proteinase K pre-treatmentfor antigen retrieval is recommended. SuggestedPpositive Control: Human tonsil. Has been described to work in FACS and Dot Blots.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocole: Protocol with Frozen, ice-cold Acetone-Fixed Sections: (The whole procedure is performed at room temperature)1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in 0.5 mL distilled water.

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.2 containing 5 mg/mL BSA as a stabilizer and 0.09 % Sodium Azide as a preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody uundiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Should this product contain a precipitate we recommend microcentrifugation before use.

Détails sur S100A9

Antigène: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Autre désignation: S100A9 / Calgranulin-B / MRP14 (S100A9 Produits)

Synonymes: anticorps 60B8AG, anticorps CAGB, anticorps CFAG, anticorps CGLB, anticorps L1AG, anticorps LIAG, anticorps MAC387, anticorps MIF, anticorps MRP14, anticorps NIF, anticorps P14, anticorps Mrp14, anticorps S100A9, anticorps MRP-14, anticorps p14, anticorps 60B8Ag, anticorps AW546964, anticorps BEE22, anticorps Cagb, anticorps GAGB, anticorps L1Ag, anticorps S100 calcium binding protein A9, anticorps S100 calcium binding protein A9 (calgranulin B), anticorps S100A9, anticorps S100a9

Sujet: S100A9 is a member of the S100 family of proteins. S100A9, together with S100A8 forms a heterodimeric protein complex, Calprotectin, which is a major calcium- and zinc-binding protein in the cytosol of neutrophils, monocytes, and keratinocytes. Complexes of S100A8 and S100A9 are the physiologically relevant forms of these proteins. S100A9 may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis. Its expression and potential cytokine-like function in inflammation and in cancer suggest that S100A8/A9 may play a key role in inflammation-associated cancer.Synonyms: CAGB, Calprotectin L1H subunit, Leukocyte L1 complex heavy chain, MRP-14, Migration inhibitory factor-related protein 14, S100 calcium-binding protein A9, S100-A9

ID gène: 9606

UniProt: P06702

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins