CD14 anticorps

N° du produit ABIN111907

Cet anticorps Souris Monoclonal détecte spécifiquement CD14 dans FACS, IHC (p) et IHC (fro). Il présente une réactivité envers Humain.

Aperçu rapide pour CD14 anticorps (ABIN111907)

Antigène: CD14

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD14 est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: X-8

Détail du produit anti-CD14 anticorps

Specificité: Monoclonal antibody X-8 prevents the Lipopolysaccharide Binding Protein (LBP) independent activation of cells via soluble CD14 by blocking the binding of CD14. BM4034 is specific for Human CD14 on monocytes, some populations of macrophages and granulocytes. The epitope has not been further characterized.

Purification: Affinity Chromatography.

Immunogène: Human Monocytes.

Isotype: IgG2b

Information d'application

Indications d'application: Immunohistochemistry on Frozen Sections: 2-4 μg/mL (1/50-1/100). Does not react on routinely processed Paraffin Sections. Recommended Positive Control: Human placenta. Has been described to work in FACS (post fixation is recommended).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore by adding 0.5 mL distilled water (= Stock solution).

Concentration: 0.2 mg/mL

Buffer: PBS pH 7.2 containing 10 mg/mL BSA as stabilizer and 0.01 % Thimerosal as preservative.

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: This product is photosensitive and should be protected from light

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted Prior to and Following Reconstitution at 2-8 °C. DO NOT FREEZE!

Détails sur CD14

Antigène: CD14

Autre désignation: CD14

Sujet: CD14 is a 53'000 - 55'000 Mr glycoprotein strongly expressed on the surface of monocytes and weakly on the surface of granulocytes. It is anchored to the surface by linkage to glycosyl phosphatidylinositol (GPI). CD14 functions as a receptor for endotoxin and is the major receptor involved in the lethal response to both endotoxin and Gram-negative bacteria. In addition to membrane CD14, soluble forms of CD14 can be detected in serum and tissue culture supernatants of cells transfected with CD14.Synonyms: Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein

ID gène: 929, 9606

UniProt: P08571

Pathways: Signalisation TLR, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades