CD14 anticorps (Biotin)

N° du produit ABIN111908

L’anticorps Souris Monoclonal anti-CD14 a été validé pour FACS et IHC (fro). Il convient pour détecter CD14 dans des échantillons de Humain.

Aperçu rapide pour CD14 anticorps (Biotin) (ABIN111908)

Antigène: CD14

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD14 est conjugé à/à la Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: X-8

Détail du produit anti-CD14 anticorps (Biotin)

Purification: Affinity chromatography.

Isotype: IgG2b

Information d'application

Indications d'application: Suitable for Immunohistochemistry (IHC) on frozen sections (0.25-0.5 μg/mL,1/800-1/1600) and FACS. Suggested positive control: Human placenta. Antigen Distribution on Isolated cells and Tissue sections: Monocytes, granulocytes, macrophages in myeloid leukaemia. Cell Lines: HL-60 without stimulation negativeHL-60 stimulated with PMA 15 % positiveHL-60 stimulated with DMSO 15 % positiveU937 negativeX-8 is positive on Langerhans cells, follicular dendritic cells, histiocytes and highendothelial cell venules. Functional assays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 0.5 mL distilled water.

Concentration: 0.4 mg/mL

Buffer: PBS, pH 7.2 with 5 mg/mL bovine serum albumin (BSA) as a stabilizer without preservatives.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody at -20 °C. This product should be stored undiluted.

Détails sur CD14

Antigène: CD14

Autre désignation: CD14

Sujet: CD14 is a 53'000 - 55'000 Mr glycoprotein strongly expressed on the surface of monocytes and weakly on the surface of granulocytes. It is anchored to the surface by linkage to glycosyl phosphatidylinositol (GPI). CD14 functions as a receptor for endotoxin and is the major receptor involved in the lethal response to both endotoxin and Gram-negative bacteria. In addition to membrane CD14, soluble forms of CD14 can be detected in serum and tissue culture supernatants of cells transfected with CD14.Synonyms: Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein

ID gène: 9606

UniProt: P08571

Pathways: Signalisation TLR, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades