ITGA2 anticorps

N° du produit ABIN111931

Cet anticorps Souris Monoclonal détecte spécifiquement ITGA2 dans IHC (fro). Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour ITGA2 anticorps (ABIN111931)

Antigène: ITGA2 (Integrin alpha 2 (ITGA2))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ITGA2 est non-conjugé

Application: Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: A-1-43

Détail du produit anti-ITGA2 anticorps

Specificité: The antibody A.1.43 detects CD49b and is a useful tumor progression marker, particularly also in combination with clone A.1.43 detects CD49b and is a useful tumour progression marker, particularly also in combination with clone A10-33/1 (anti gpIIb/IIIa). CD49b, integrin α2, is a transmembrane glycoprotein that is non-covalently associated with the integrin β1 chain to form the α2β1 (very late antigen (VLA) 2) complex. The α2β1 complex was originally reported on long-term activated T cells and was later shown to be identical to the gpIa/IIa complex on platelets. The α2β1 complex is a pivotal receptor for collagen, and the adhesion of platelets to collagen is known to be important for the initial step of platelet aggregation. The antibody stains an epitope on the cell surface. The antibody is positive on the following melanoma cell lines: BRO, SK-Mel-13, SK-Mel-19, SK-Mel-29, SK-Mel-113, and negative on normal monocytes. Tissue sections: Acetone fixed tissue sections of melanoma larger than 1.5 mm show positive staining whereas the majority of benign melanocytic nevi (>95 %) are negative.

Purification: Affinity chromatography.

Isotype: IgG1

Information d'application

Indications d'application: Suitable for Immunohistochemistry on frozen sections (0.25-2 μg/mL, 1/100-1/800) andFACS. Suggested positive control: Human skin. The antibody is positive on the following melanoma cell lines: BRO, SK-Mel-13, SK-Mel-19,SK-Mel-29, SK-Mel-113, and negative on normal monocytes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocole: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum For further information and details see technicalinformation

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 0.6 mL distilled water.

Concentration: 0.2 mg/mL

Buffer: PBS buffer pH 7.2 with 0.01 % Thimerosal as preservative and 1 % BSA as stabilizer.

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Détails sur ITGA2

Antigène: ITGA2 (Integrin alpha 2 (ITGA2))

Autre désignation: CD49b / ITGA2

Sujet: Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits, which mediate cell-cell and cell-extracellular matrix attachments. They are responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. Aberrant integrin expression has been found in many epithelial tumours. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells.Synonyms: CD49 antigen-like family member B, Collagen Receptor, GPIa, ITGA-2, Integrin alpha-2, Platelet membrane glycoprotein Ia, VLA-2 alpha, VLA2

ID gène: 3673

NCBI Accession: NP_002194

UniProt: P17301

Pathways: CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Integrin Complex