CD4 anticorps

N° du produit ABIN111996

Cet anticorps anti-CD4 est un anticorps Souris Monoclonal détectant CD4 dans FACS, IHC (fro) et Func. Adapté pour Rat.

Aperçu rapide pour CD4 anticorps (ABIN111996)

Antigène: CD4

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD4 est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Clone: Rib 5-2

Détail du produit anti-CD4 anticorps

Purification: Affinity chromatography.

Immunogène: Lymphoblasts.

Isotype: IgG2a

Information d'application

Indications d'application: Suitable for Immunohistochemistry (IHC), has been described to work in FACS (see Table1). Recommended Dilutions for IHC: Frozen sections: 1.5 μg/mL (1/200)Paraffin sections: does not react on routinely processed paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocole: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG (H+L)minimal-cross reaction to rat) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 0.5 mL distilled water.

Concentration: 0.3 mg/mL

Buffer: PBS, pH 7.2 containing 0.01 % Thiomerosal as preservative and 10 mg/mL BSA as stabilizer.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody at 2-8 °C for one month or at -20 °C for longer.

Détails sur CD4

Antigène: CD4

Autre désignation: CD4

Sujet: CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

ID gène: 10116

UniProt: P05540

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events