CD14 anticorps

N° du produit ABIN112001

L’anticorps anti-CD14 Monoclonal produit chez le Souris a été validé pour FACS et IHC (fro). Il convient pour la détection de CD14 dans des échantillons provenant de Humain. }

Aperçu rapide pour CD14 anticorps (ABIN112001)

Antigène: CD14

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD14 est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: Mab0211

Détail du produit anti-CD14 anticorps

Purification: Affinity chromatography.

Isotype: IgG2a

Information d'application

Indications d'application: Suitable for Immunohistochemitry (IHC) on frozen sections: 1 μg/mL (1: 200)and Facs. Antigen Distribution: Isolated cells: Positive on granulocytes and monocytes, myeloid leukemias and myeloidcell lines. Tissue sections: Positive on histocytes, Langerhans cells, follicular dendritic cells and highendothelial venules.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocole: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum For further information and details see technicalinformation

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 0.5 mL distilled water

Buffer: Phosphate buffered saline, pH 7.2 with 0.02 % sodium azide as preservative and 1 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Dilute only prior to immediate use

Stock: 4 °C

Stockage commentaire: Store the antibody at 2-8 °C as an undiluted liquid. DO NOT FREEZE! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Détails sur CD14

Antigène: CD14

Autre désignation: CD14

Sujet: CD14 is a 53'000 - 55'000 Mr glycoprotein expressed strongly on the surface of monocytes and weakly on the surface of granulocytes. It is anchored to the surface by linkage to glycosyl phosphatidylinositol (GPI). CD14 functions as a receptor for endotoxin and is the major receptor involved in the lethal response to both endotoxin and Gram-negative bacteria. In addition to membrane CD14, soluble forms of CD14 can be detected in serum and tissue culture supernatants of cells transfected with CD14.Synonyms: Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein

ID gène: 9606

UniProt: P08571

Pathways: Signalisation TLR, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades