KIT Ligand anticorps (Extracellular Domain)

N° du produit ABIN112014

Cet anticorps anti-KIT Ligand est un anticorps Souris Monoclonal détectant KIT Ligand dans WB, EIA, IHC (p) et IHC (fro). Adapté pour Humain et Primate.

Aperçu rapide pour KIT Ligand anticorps (Extracellular Domain) (ABIN112014)

Antigène: KIT Ligand (KITLG)

Reactivité: Humain, Primate

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp KIT Ligand est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: HKL12

Détail du produit anti-KIT Ligand anticorps

Épitope: Extracellular Domain

Specificité: This Monoclonal antibody hKL12 is specific for Stem Cell Factor (hSCF). hKL12 is useful for the detection of hSCF in tissues, isolated cells and for studying biological effects of Human Stem Cell Factor in vitro. Antigen distribution on Tissue sections: Bone marrow stromal cells, fibroblasts, and fetal liver cells express SCF. Keratinocytes stain positive.

Réactivité croisée (Details): Species reactivity (expected):Primates.
Species reactivity (tested):Human.

Purification: Affinity Chromatography

Immunogène: Recombinant Human stem cell factor. Remarks: Recombinant Human SFC is an 18.4 kDa polypeptide containing 165 amino acid residues, corresponding to the sequence of the soluble form of SCF. The antigen is a 248 residue protein. The full-length transcript of the corresponding gene product includes a 25-amino acid secretory leader, a 189-amino acid extracellular domain, a 23-amino acid transmembrane region, and a 36-amino acid cytoplasmic tail. The epitope has not been further characterized.

Isotype: IgG1

Information d'application

Indications d'application: ELISA (0.15 μg/mL). Has been described to work in Wetsern blots and Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore by adding 0.5 mL distilled water.

Concentration: 0.4 mg/mL

Buffer: PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Détails sur KIT Ligand

Antigène: KIT Ligand (KITLG)

Autre désignation: KITLG / SCF

Sujet: SCF is a transmembrane protein expressed as two alternatively spliced isoforms, either 220 or 248 amino acids, the former lacking a peptidase cleavage site. The cleavage site is responsible for the release of the soluble form of the protein from the cell surface. The first 164 or 165 residues of the extracellular domain comprise soluble SCF, which exists in solution as a noncovalently linked homodimer. Alone, SCF is a weakly proliferative stimulus but acts synergistically with numerous cytokines. SCF is important in germ cell developement, and an indispensable facor for mast cell proliferation an differentiation.Synonyms: KITL, Kit ligand, MGF, Mast cell growth factor, Stem cell factor, c-Kit ligand

ID gène: 4254

NCBI Accession: NP_000890

UniProt: P21583

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway