KIT Ligand anticorps (Extracellular Domain)
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- Antigène Voir toutes KIT Ligand (KITLG) Anticorps
- KIT Ligand (KITLG)
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Épitope
- Extracellular Domain
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Reactivité
- Humain, Primate
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp KIT Ligand est non-conjugé
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Application
- Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Specificité
- This Monoclonal antibody hKL12 is specific for Stem Cell Factor (hSCF). hKL12 is useful for the detection of hSCF in tissues, isolated cells and for studying biological effects of Human Stem Cell Factor in vitro. Antigen distribution on Tissue sections: Bone marrow stromal cells, fibroblasts, and fetal liver cells express SCF. Keratinocytes stain positive.
- Réactivité croisée (Details)
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Species reactivity (expected):Primates.
Species reactivity (tested):Human. - Purification
- Affinity Chromatography
- Immunogène
- Recombinant Human stem cell factor. Remarks: Recombinant Human SFC is an 18.4 kDa polypeptide containing 165 amino acid residues, corresponding to the sequence of the soluble form of SCF. The antigen is a 248 residue protein. The full-length transcript of the corresponding gene product includes a 25-amino acid secretory leader, a 189-amino acid extracellular domain, a 23-amino acid transmembrane region, and a 36-amino acid cytoplasmic tail. The epitope has not been further characterized.
- Clone
- HKL12
- Isotype
- IgG1
- Top Product
- Discover our top product KITLG Anticorps primaire
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- Indications d'application
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ELISA (0.15 μg/mL). Has been described to work in Wetsern blots and Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Reconstitution
- Restore by adding 0.5 mL distilled water.
- Concentration
- 0.4 mg/mL
- Buffer
- PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.
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- Antigène
- KIT Ligand (KITLG)
- Autre désignation
- KITLG / SCF (KITLG Produits)
- Synonymes
- anticorps Xkl-1, anticorps Xsl, anticorps Xsl-1, anticorps Xsl-2, anticorps steel, anticorps KITLG, anticorps SCF, anticorps kitl, anticorps kl-1, anticorps mgf, anticorps scf, anticorps FPH2, anticorps KL-1, anticorps Kitl, anticorps MGF, anticorps SF, anticorps SHEP7, anticorps Clo, anticorps Con, anticorps Gb, anticorps Kitlg, anticorps Mgf, anticorps SLF, anticorps Sl, anticorps blz, anticorps contrasted, anticorps 710-712, anticorps CSF, anticorps KITL, anticorps KIT ligand L homeolog, anticorps KIT ligand, anticorps kit ligand, anticorps kitlg.L, anticorps KITLG, anticorps kitlg, anticorps Kitl, anticorps Kitlg
- Sujet
- SCF is a transmembrane protein expressed as two alternatively spliced isoforms, either 220 or 248 amino acids, the former lacking a peptidase cleavage site. The cleavage site is responsible for the release of the soluble form of the protein from the cell surface. The first 164 or 165 residues of the extracellular domain comprise soluble SCF, which exists in solution as a noncovalently linked homodimer. Alone, SCF is a weakly proliferative stimulus but acts synergistically with numerous cytokines. SCF is important in germ cell developement, and an indispensable facor for mast cell proliferation an differentiation.Synonyms: KITL, Kit ligand, MGF, Mast cell growth factor, Stem cell factor, c-Kit ligand
- ID gène
- 4254
- NCBI Accession
- NP_000890
- UniProt
- P21583
- Pathways
- Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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