Desmoplakin anticorps

N° du produit ABIN112137

Cet anticorps anti-Desmoplakin Monoclonal Souris (Clone DP-2-17) (ABIN112137) détecte spécifiquement Desmoplakin dans WB, IHC (fro) et IHC (p). L’anticorps est réactif avec des échantillons de Humain, Souris, Rat, Poulet et Boeuf (Vache).

Aperçu rapide pour Desmoplakin anticorps (ABIN112137)

Antigène: Desmoplakin (DSP)

Reactivité: Humain, Souris, Rat, Poulet, Boeuf (Vache)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Desmoplakin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: DP-2-17

Détail du produit anti-Desmoplakin anticorps

Specificité: DP 2.17 shows distinct punctate membrane staining of epithelia. It reacts with the rod fragment of Desmoplakin 1 (Mr 250,000 polypeptide of desmosomal plaques). Tested Reactivities on Cultured Cell Lines: Several Human carcinoma cell lines: MCF-7, A-431, TR-146. Bovine cells: MDBK, BMGE.

Réactivité croisée (Details): Species reactivity (tested):Human, Bovine, Rat, Chicken and Mouse.

Purification: Protein A Affinity Chromatography

Immunogène: Bovine Desmoplakin 1

Isotype: IgG1

Information d'application

Indications d'application: Immunohistochemistry on frozen and paraffin embedded sections after microwavetreatment (dilute 1: 10 with PBS pH 7.4). Western blot (Incubation time: 1h at RT).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 1 mL distilled water.

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide and 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Prior to reconstitution store at -70 °C. Following reconstitution store the antibody (in aliquots) at -20 °C for 6 month.

Date de péremption: 6 months

Détails sur Desmoplakin

Antigène: Desmoplakin (DSP)

Autre désignation: Desmoplakin

Sujet: Desmosomes are intercellular junctions that form tight links between adjacent cells. Desmoplakin is an obligate component of functional desmosomes that attaches intermediate filaments to desmosomal plaques. It is involved in the organization of desmosomal cadherin-plakoglobin complexes into discrete plasma membrane domains. The N-terminus of desmoplakin is essential for localisation to the desmosome and interaction with plakophilin 1 and plakoglobin. The C-terminus of desmoplakin binds to intermediate filaments. The central region of desmoplakin comprises a coiled-coil rod domain that mediates homodimerisation. There are two isoforms of desmoplakin - desmoplakin I, which is an obligate component of all desmosomes, and desmoplakin II, which is predominantly expressed in tissues and cells of stratified origin. Mutations in the gene encoding desmoplakin result in a number of cardiomyopathies and keratodermas as well as the autoimmune disease paraneoplastic pemphigus.Synonyms: 250/210 kDa paraneoplastic pemphigus antigen, DP, DSP

ID gène: 1832

NCBI Accession: NP_001008844

UniProt: P15924

Pathways: Cell-Cell Junction Organization