CDK4 anticorps

N° du produit ABIN112138

Cet anticorps Souris Monoclonal détecte spécifiquement CDK4 dans WB, IHC (p) et IHC (fro). Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour CDK4 anticorps (ABIN112138)

Antigène: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CDK4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: DCS-156

Détail du produit anti-CDK4 anticorps

Specificité: DCS-35 reacts specifically with CDK4 protein present predominantly in G1 phase of cell cycle. It does not cross-react with cyclins. In immunohisto-chemical application on frozen sections of head and neck carcinoma (oropharynx and hypopharynx carcinoma) DCS-156 shows distinct nuclear staining, especially in tumor areas of enhanced proliferation.

Purification: Affinity Chromatohgraphy on Protein A.

Immunogène: Human recombinant full-length cdk4 polypeptide.

Isotype: IgG1

Information d'application

Indications d'application: Immunoblotting (Western blotting). Suitable for Immunohistochemistry on Frozen and Paraffin Embedded Sections (improvedstaining after microwave treatment): 1/10Incubation Time: 1-4 h at RT or overnight at 2-8 °C.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in 1 mL distilled water.

Conseil sur la manipulation: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur CDK4

Antigène: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Autre désignation: CDK4

Sujet: Cdk4 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. Cdk4 is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1/S phase, which is controlled by the regulatory subunits D type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). The mutations in this gene as well as its related proteins including D type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Two alternatively spliced variants, and multiple polyadenylation sites of this gene have been reported.Synonyms: Cell division protein kinase 4, Cyclin-dependent kinase 4, PSK-J3

ID gène: 1019

NCBI Accession: NP_000066

UniProt: P11802

Pathways: Cycle Cellulaire, Mitotic G1-G1/S Phases, Regulation of Cell Size