CDK4 anticorps

N° du produit ABIN112142

L’anticorps Souris Monoclonal anti-CDK4 a été validé pour WB et IP. Il convient pour détecter CDK4 dans des échantillons de Humain, Souris, Rat et Porc.

Aperçu rapide pour CDK4 anticorps (ABIN112142)

Antigène: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Reactivité: Humain, Souris, Rat, Porc

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CDK4 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: DCS-35

Détail du produit anti-CDK4 anticorps

Specificité: This antibody reacts specifically with cdk4 protein present predominantly in G1 phase of cell cycle, it does not cross-react with cyclins. Especially suitable for immunoprecipitation, mab DCS-35 precipitates the cdk4/cyclin complex.

Purification: Protein A affinity chromatography

Immunogène: Human recombinant full-length cdk4 polypeptide

Isotype: IgG1

Information d'application

Indications d'application: Immunoprecipitation: 1: 3 - 1: 10, 1 - 4 h at RT or overnight at 2-8C. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in 1 mL dist. water

Buffer: PBS buffer, pH 7.4 containing 0.09 % NaN3, 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur CDK4

Antigène: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Autre désignation: CDK4

Sujet: Cdk4 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. Cdk4 is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1/S phase, which is controlled by the regulatory subunits D type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). The mutations in this gene as well as its related proteins including D type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Two alternatively spliced variants, and multiple polyadenylation sites of this gene have been reported.Synonyms: Cell division protein kinase 4, Cyclin-dependent kinase 4, PSK-J3

ID gène: 1019

NCBI Accession: NP_000066

UniProt: P11802

Pathways: Cycle Cellulaire, Mitotic G1-G1/S Phases, Regulation of Cell Size