ADRP anticorps (N-Term)

N° du produit ABIN112185

Détail du produit anti-ADRP anticorps

Antigène: ADRP (PLIN2) (Perilipin 2 (PLIN2))

Épitope: AA 5-27, N-Term

Reactivité: Humain, Rat, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ADRP est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: Polypeptide Reacting: Adipophilin / ADRP, MW 48,100 (calculated from aa sequence data), apparent Mr 52,000 (after SDS-PAGE), pI 6.72. Tissue Immunolocalization: Adipophilin is positively detected in the glandular cells of lactating mammary gland (ductal cells are negative), zona fasciculata of the adrenal gland, Sertoli cells of the testis, and in fat-accumulating hepatocytes of alcoholic cirrhotic fatty liver, adipocytes are negative. Also positively stained are lipid-storing CD 68-positive macrophages. Tested Reactivity on Cultured Cell lines: Caco, PLC, HaCat, SV80, RD 125, Huvec (human umbilical cord endothelia), RV, PC-12 (rat adrenal gland), MDCK. Negative with glioma.

Réactivité croisée (Details): Species reactivity (tested):Human, Rat and Dog. Negative with Bovine and Mouse.

Purification: Affinity Chromatography

Immunogène: Synthetic peptide corresponding to aa 5-27 from N-terminus of Human Adipophilin.

Clone: AP125

Isotype: IgG1

Information d'application

Indications d'application: Immunohistochemistry on Frozen Tissue: 1/10 in PBS, pH 7.4(See Ref.17 for staining protocols). Immunohistochemistry on Paraffin-Embedded Tissue (After microwave treatment). Incubate 1 h at room temperature or over night at 2-8 °C. Cytological Material. Immunoblotting (Western blot): 0.2 μg/mL using ECL has been reported for previous batchnumbers.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 1.0 mL distilled water

Buffer: Final solution contains 0.09 % Sodium Azide and 0.5 % BSA in PBS buffer, pH 7.4

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store the lyophilized antibody at -20 °C.

Références pour anticorps anti-ADRP (ABIN112185)

Leitner, Hlavatý, Ertl, Gabner, Fuchs-Baumgartinger, Walter: "Lipid droplets and perilipins in canine osteosarcoma. Investigations on tumor tissue, 2D and 3D cell culture models." dans: Veterinary research communications, Vol. 46, Issue 4, pp. 1175-1193, (2022) (PubMed).

Leitner, Hlavaty, Heider, Ertl, Gabriel, Walter: "Lipid droplet dynamics in healthy and pyometra-affected canine endometrium." dans: BMC veterinary research, Vol. 18, Issue 1, pp. 221, (2022) (PubMed).

Breher-Esch, Sahini, Trincone, Wallstab, Borlak: "Genomics of lipid-laden human hepatocyte cultures enables drug target screening for the treatment of non-alcoholic fatty liver disease." dans: BMC medical genomics, Vol. 11, Issue 1, pp. 111, (2019) (PubMed).

Skwarek-Maruszewska, Hotulainen, Mattila, Lappalainen: "Contractility-dependent actin dynamics in cardiomyocyte sarcomeres." dans: Journal of cell science, Vol. 122, Issue Pt 12, pp. 2119-26, (2009) (PubMed).

Ohsaki, Maeda, Fujimoto: "Fixation and permeabilization protocol is critical for the immunolabeling of lipid droplet proteins." dans: Histochemistry and cell biology, Vol. 124, Issue 5, pp. 445-52, (2005) (PubMed).

Détails sur ADRP

Antigène: ADRP (PLIN2) (Perilipin 2 (PLIN2))

Autre désignation: Adipophilin / ADFP (PLIN2 Produits)

Synonymes: anticorps ADFP, anticorps ADRP, anticorps AA407157, anticorps ADPH, anticorps Adfp, anticorps Adrp, anticorps adfp, anticorps adrp, anticorps fatvg, anticorps MGC76047, anticorps adipophilin, anticorps PLIN2, anticorps DKFZp469F1012, anticorps perilipin-2, anticorps zgc:110411, anticorps perilipin 2, anticorps perilipin 2 L homeolog, anticorps adipophilin, anticorps perilipin-2, anticorps PLIN2, anticorps Plin2, anticorps plin2, anticorps plin2.L, anticorps LOC100304704, anticorps LOC100380740

Sujet: Adipophilin / ADRP is a ubiquitous component of lipid droplets. It has been found in milk fat globule membranes and on the surface of lipid droplets in various cultured cell lines (see e.g. Heid et al., for review see e.g. Targett-Adams et al.), inducible by etomoxir. Enhanced expression of Adipophilin / ADRP is a useful marker for pathologies characterized by increased lipid droplet accumulation. Such diseases include atheroma, steatosis, obesity and certain cases of liposarcoma. It also seems to be a potent marker for atherosclerosis. ADRP can also be used to study the virus entry of e.g. HCV via lipid droplets (see e.g. Hope et al.).Synonyms: ADRP, Adipose differentiation-related protein

ID gène: 123

NCBI Accession: NP_001113

UniProt: Q99541

Pathways: Regulation of Lipid Metabolism by PPARalpha, Lipid Metabolism