CD3 anticorps (FITC)

N° du produit ABIN114140

L’anticorps Hamster Monoclonal anti-CD3 a été validé pour FACS. Il convient pour détecter CD3 dans des échantillons de Souris.

Aperçu rapide pour CD3 anticorps (FITC) (ABIN114140)

Antigène: CD3

Reactivité: Souris

Hôte: Hamster

Clonalité: Monoclonal

Conjugué: Cet anticorp CD3 est conjugé à/à la FITC

Application: Flow Cytometry (FACS)

Clone: 500-A2

Détail du produit anti-CD3 anticorps (FITC)

Specificité: This anti-mouse CD3ε monoclonal antibody is specific for the ε chain of mouse CD31. Immobolized 500A2 mAb can be used to stimulate mouse T cells in vitro1. A combination of immobolized 500A2 mAb and anti-mouse CD28 mAb is commonly used for the activation of mouse T cells3.

Immunogène: C6VL-BS cell lysate

Isotype: IgG

Information d'application

Indications d'application: Flow Cytometry (see Protocols). (Reportet to be useful in immunoprecipitation,immunofluorescent staining and immunohistochemistry on acetone fixed frozen sections. )
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocole: FLOW CYTOMETRY ANALYSIS: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test).

Restrictions: For Research Use only

Stockage

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % NaN3 and EIA grade BSA as stabilizing protien to bring total protein concentration to 4-5 mg/mL

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur CD3

Antigène: CD3

Autre désignation: CD3

Sujet: In the mouse, CD3 is highly expressed by mature T cells and at lower levels by CD4+CD8+ thymocytes2. The ε chain of CD3 is a component of the multimeric T cell receptor complex and is involved with signal transductionSynonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4

ID gène: 12501

NCBI Accession: NP_031674

UniProt: P22646

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway