KIT anticorps (FITC)
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- Antigène Voir toutes KIT Anticorps
- KIT (Mast/stem Cell Growth Factor Receptor (KIT))
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Reactivité
- Souris
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Hôte
- Rat
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp KIT est conjugé à/à la FITC
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Application
- Flow Cytometry (FACS)
- Specificité
- Recognizes the receptor tyrosine kinase, c-kit (CD117). c-kit positive cells are a subset of CD34+ hematopoietic precursor cells and it is expressed on 5-10 % of total adult bone marrow cells.
- Attributs du produit
- Absorption / Emission: 495 nm / 528 nm
- Purification
- Protein G Chromatography.
- Immunogène
- IL-3 dependent mast cells derived from WB- +/+ mice. Donor: Wistar spleen. Fusion Partner: X63.653. Ag8.
- Clone
- ACK4
- Isotype
- IgG2a
- Top Product
- Discover our top product KIT Anticorps primaire
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- Indications d'application
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Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Protocole
- Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add 1 µg* of ABIN114257 per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes are protected from light, since most fluorochromes arelight sensitive. )7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 µl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media:
- Restrictions
- For Research Use only
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- Concentration
- 0.1 mg/mL
- Buffer
- PBS containing 0.02 % Sodium Azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Antigène
- KIT (Mast/stem Cell Growth Factor Receptor (KIT))
- Autre désignation
- CD117 / C-Kit (KIT Produits)
- Synonymes
- anticorps C-Kit, anticorps CD117, anticorps PBT, anticorps SCFR, anticorps Bs, anticorps Fdc, anticorps Gsfsco1, anticorps Gsfsco5, anticorps Gsfsow3, anticorps SCO1, anticorps SCO5, anticorps SOW3, anticorps Ssm, anticorps Tr-kit, anticorps W, anticorps c-KIT, anticorps c-kit, anticorps MGF, anticorps KIT, anticorps C-KIT, anticorps kit, anticorps receptor, anticorps sparse, anticorps Xkl-1, anticorps kit-a, anticorps kit-b, anticorps kl1-A, anticorps krk1, anticorps pbt, anticorps scfr, anticorps KIT proto-oncogene receptor tyrosine kinase, anticorps v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog a, anticorps KIT proto-oncogene receptor tyrosine kinase S homeolog, anticorps KIT, anticorps Kit, anticorps kita, anticorps kit, anticorps kit.S
- Sujet
- CD117 or c-kit is a receptor tyrosine kinase. The ligand for this receptor is steel factor (stem cell factor), which exists in both soluble and membrane form. The interaction between steel factor and c-kit is essential for the development of hematopoietic, gonadal and pigment stem cells.Synonyms: KIT, Mast/stem cell growth factor receptor, Proto-Oncogene Tyrosine-Protein Kinase, SCFR
- ID gène
- 16590
- NCBI Accession
- NP_066922
- UniProt
- P05532
- Pathways
- Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity
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