Clusterin anticorps

N° du produit ABIN114700

Détail du produit anti-Clusterin anticorps

Antigène: Clusterin (CLU)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Clusterin est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: The anti-Clusterin Antibody, Clone Hs-3 is a Mouse monoclonal antibody against Human Clusterin. Does not react with Cat, Dog, Bovine.

Purification: Affinity chromatography on protein A followed by DEAE-chromatography

Immunogène: The antibody was prepared against Triton X-100 human sperm extract.

Clone: Hs-3

Isotype: IgG1

Information d'application

Indications d'application: ELISA. Immunocytochemistry. Immunofluorescence. Immunohistochemistry. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Add 0.1 mL of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.

Concentration: 1.0 mg/mL

Buffer: 0.05 M Phosphate buffer, 0.5 M NaCl, pH 7.2, 15 mM Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Prior to reconstitution store at -70 °C. Following reconstitution store the antibody (in aliquots) at -20 °C for 6 month.

Date de péremption: 6 months

Références pour anticorps anti-Clusterin (ABIN114700)

Steinke, Dally, Friedel, Walles, Walles: "Host-integration of a tissue-engineered airway patch: two-year follow-up in a single patient." dans: Tissue engineering. Part A, Vol. 21, Issue 3-4, pp. 573-9, (2015) (PubMed).

Schlötzer-Schrehardt, Pasutto, Sommer, Hornstra, Kruse, Naumann, Reis, Zenkel: "Genotype-correlated expression of lysyl oxidase-like 1 in ocular tissues of patients with pseudoexfoliation syndrome/glaucoma and normal patients." dans: The American journal of pathology, Vol. 173, Issue 6, pp. 1724-35, (2008) (PubMed).

Zhang, Sha, Wood, Galindo, Garner, Burkart, Suarez, Sierra, Agar, Peterson, Chopra: "Alteration in the activation state of new inflammation-associated targets by phospholipase A2-activating protein (PLAA)." dans: Cellular signalling, Vol. 20, Issue 5, pp. 844-61, (2008) (PubMed).

Kilic, Oliveira-Ferrer, Neshat-Vahid, Irmak, Obst-Pernberg, Wurmbach, Loges, Kilic, Weil, Lauke, Tilki, Singer, Ergün: "Lymphatic reprogramming of microvascular endothelial cells by CEA-related cell adhesion molecule-1 via interaction with VEGFR-3 and Prox1." dans: Blood, Vol. 110, Issue 13, pp. 4223-33, (2007) (PubMed).

Schenke-Layland, Madershahian, Riemann, Starcher, Halbhuber, König, Stock: "Impact of cryopreservation on extracellular matrix structures of heart valve leaflets." dans: The Annals of thoracic surgery, Vol. 81, Issue 3, pp. 918-26, (2006) (PubMed).

Détails sur Clusterin

Antigène: Clusterin (CLU)

Autre désignation: Apolipoprotein J (Apo J) (CLU Produits)

Synonymes: anticorps LOC100136120, anticorps clu, anticorps MGC53055, anticorps zgc:73352, anticorps xclu, anticorps MGC89163, anticorps CLU, anticorps APO-J, anticorps APOJ, anticorps CLI, anticorps CLU1, anticorps CLU2, anticorps KUB1, anticorps NA1/NA2, anticorps SGP-2, anticorps SGP2, anticorps SP-40, anticorps TRPM-2, anticorps TRPM2, anticorps DAG, anticorps RATTRPM2B, anticorps SP40, anticorps TRPM2B, anticorps Trpm2, anticorps Trpmb, anticorps AI893575, anticorps ApoJ, anticorps Cli, anticorps D14Ucla3, anticorps Sgp-2, anticorps Sgp2, anticorps Sugp-2, anticorps GP80, anticorps gpIII, anticorps 40, anticorps clusterin-1, anticorps clusterin L homeolog, anticorps clusterin, anticorps Clusterin, anticorps LOC100136120, anticorps clu.L, anticorps clu, anticorps CLU, anticorps clus, anticorps Clu, anticorps LOC101113728

Sujet: Clusterin is a 75-80 kD disulfide-linked heterodimeric protein containing about 30 % of N-linked carbohydrate rich in sialic acid, but truncated forms targeted to the nucleus have also been identified. The precursor polypeptide chain is cleaved proteolytically to remove the 22-mer secretory signal peptide and subsequently between residues 227/228 to generate the alpha and beta chains. These are assembled antiparallel to give a heterodimeric molecule in which the cysteine-rich centers are linked by five disulfide bridges and are flanked by two predicted coiled-coil alpha-helices and three predicted amphipathic alpha-helices. The six sites of N-linked glycosylation are indicated as yellow spots. Across a broad range of species clusterin shows 70 % to 80 % of sequence homology. It is ubiquitously expressed in most mammalian tissues and can be found in plasma, milk, urine, cerebrospinal fluid and semen. It is able to bind and form complexes with numerous partners such as immunoglobulins, lipids, heparin, bacteria, complement components, paraoxonase, beta amyloid, leptin and others. Clusterin has been ascribed a plethora of functions such as phagocyte recruitment, aggregation induction, complement attack prevention, apoptosis inhibition, membrane remodelling, lipid transport, hormone transport and/or scavenging, matrix metalloproteinase inhibition. A detailed mechanism of clusterin has not been defined. One tempting hypothesis says that clusterin is an extracellular chaperone protecting cells from stress induced by degraded and misfolded protein precipitates.Clusterin is up- or downregulated on the mRNA or protein level in many pathological and clinically relevant situations including cancer, organ regeneration, infection, Alzheimer disease, retinitis pigmentosa, myocardial infarction, renal tubular damage, autoimmunity and others.Synonyms: 40, 40, AAG4, APOJ, Aging-associated gene 4 protein, CLI, Clusterin, Complement cytolysis inhibitor, Complement-associated protein SP-40, KUB1, Ku70-binding protein 1, NA1/NA2, SP-40, Testosterone-repressed prostate message 2

ID gène: 1191

NCBI Accession: NP_976084

UniProt: P10909

Pathways: Apoptose, Negative Regulation of intrinsic apoptotic Signaling