CCL2 anticorps

N° du produit ABIN114713

L’anticorps anti-CCL2 Monoclonal Souris est utilisé pour la détection de CCL2 dans des échantillons de Humain. Il a été validé pour WB, EIA et Func.

Aperçu rapide pour CCL2 anticorps (ABIN114713)

Antigène: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CCL2 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Clone: S101

Détail du produit anti-CCL2 anticorps

Purification: Affinity Chromatography on Protein G.

Immunogène: Purified recombinant human MCP-1

Isotype: IgG1

Information d'application

Indications d'application: ELISA: React with human MCP-1. Matched pair antibodies are available. Neutralizing: Tests on the ability to inhibit monocyte chemotaxis toward 1 nM recombinanthuman MCP-1 in blindwell chambers showed that the antibodies were particularly effectiveat blocking MCP-1 activity at a concentration of 0.5 μg/mL. It was also found that S-14 couldinhibit the function of native MCP-1 at concentrations similar to inhibitory doses forrecombinant MCP-1. Western Blot: Concentration of 0.1-1.0 μg/mL will allow visualization of 0.1 μg/lane ofhuman MCP-1. Concentration of 5.0 μg/mL will allow visualization of 50 μg/lane of rabbitMCP-1.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with Double distillated water to adjust the final concentration to 1.0 mg/mL.

Concentration: 1.0 mg/mL

Buffer: 0.01 M PBS, pH 7.2 without preservatives

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Détails sur CCL2

Antigène: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Autre désignation: MCP1 / CCL2

Sujet: Monocyte chemotactic and activating factor (MCAF) is also called monocyte chemotactic protein-1 (MCP-1) and chemokine (C-C motif) ligand 2 (CCL2). It is primarily secreted by monocytes, macrophages and dendritic cells. This cytokine displays chemotactic activity for monocytes, T-cells, and basophils, but not for neutrophils or eosinophils. MCAF causes the degranulation of basophils and mast cells, and augments the activity of monocyte and macrophage. MCAF plays an important role in inflammation, angiogenesis, autoimmune diseases, renal diseases, chronic infection and granuloma formation.Synonyms: C-C motif chemokine 2, HC-11, HC11, MCAF, MCP-1, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, SCYA2, Small-inducible cytokine A2

ID gène: 9606

UniProt: P13500

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, Phosphorylation & l'infection par le SRAS-CoV-2