HSPG2 anticorps

N° du produit ABIN114947

Détail du produit anti-HSPG2 anticorps

Antigène: HSPG2 (Heparan Sulfate Proteoglycan 2 (HSPG2))

Reactivité: Humain, Souris, Boeuf (Vache), Rat, Wolf, Bactéries, Morue

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp HSPG2 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: A7L6 recognizes domain IV of the core protein of the large Heparan Sulphate Proteoglycan or Perlecan. The reactivity is independent of the galactosaminoglycan moieties. Therefore, the epitope is not sensitive to Heparitinase treatment.

Purification: Purified

Immunogène: High molecular mass material derived from the Engelbreth-Holm-Swarm (EHS) tumor matrix containing laminin, entactin and HSPG.

Clone: A7L6

Isotype: IgG2a

Information d'application

Indications d'application: Immunoprecipitation. Immunoblotting: 1/100-1/1000. Immunocytochemistry. Immunohistochemistry on frozen and paraffin-embedded tissues: 1/25-1/200 (withavidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing. Do not freeze working dilutions.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the original vial at 2-8 °C. After reconstitution store (in aliquots) at -20 °C.

Détails sur HSPG2

Antigène: HSPG2 (Heparan Sulfate Proteoglycan 2 (HSPG2))

Autre désignation: HSPG2 / Perlecan (HSPG2 Produits)

Synonymes: anticorps HSPG, anticorps PLC, anticorps PRCAN, anticorps SJA, anticorps SJS, anticorps SJS1, anticorps HSPG2, anticorps AI852380, anticorps Pcn, anticorps Plc, anticorps per, anticorps Per, anticorps heparan sulfate proteoglycan 2, anticorps perlecan (heparan sulfate proteoglycan 2), anticorps HSPG2, anticorps hspg2, anticorps Hspg2

Sujet: Proteoglycans are macromolecules consisting of a variety of core proteins with covalently attached one or several polysaccharide chains of the glycosaminoglycan type (heparan sulphate, heparin, chondroitin sulphate, dermatan sulphate or keratan sulphate). At least two forms of basement membrane heparan sulphate proteoglycan (HSPG) have been identified. One with a large core protein (> 400 kD) and one with a small core protein (30 kD). The large HSPG is probably the most abundant basement membrane proteoglycan. It is located predominantly in the lamina lucida, where it forms clustered aggregates and interacts with other basement membrane components to form the matrix. In addition, it also plays a critical role in attachment of cells to the basal membrane via integrin receptors.Synonyms: Basement membrane-specific heparan sulfate proteoglycan core protein, PLC

ID gène: 3339

NCBI Accession: NP_005520

UniProt: P98160

Pathways: Glycosaminoglycan Metabolic Process, Lipid Metabolism