LYVE1 anticorps (AA 24-228)

N° du produit ABIN115688

Détail du produit anti-LYVE1 anticorps

Antigène: LYVE1 (Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE1))

Épitope: AA 24-228

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LYVE1 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Specificité: This antibody detetcs Lyve-1.

Réactivité croisée (Details): Species reactivity (tested):Mouse. This antibody is not reactive with Human LYVE-1.

Purification: Protein G Chromatography (+ his tag depletetion)

Immunogène: Highly pure recombinant Mouse soluble LYVE-1 produced in insect cells. This recombinant soluble LYVE-1 consists of amino acid 24 (Ala) to 228 (Gly) and is fused to a C-terminal His-tag (6xHis).

Information d'application

Indications d'application: ELISA (1-15 μg/mL). Western blot (1-2 μg/mL). FACS analysis (3-20 μg/mL). Immunohistochemistry on Frozen Sections (1-5 μg/mL). For formalin-fixed, paraffin-embedded sections use the immunogen affinity purifiedantibody ABIN115689.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile water/PBS to a concentration of > 0.5 mg/mL.

Buffer: PBS, pH 7.2 without preservatives or stabilizers

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilized IgG is stable at 2-8 °C for one month and at -20 °C for longer. When reconstituted the antibody is stable for at least six weeks at 2-8 °C. For longer store in aliquots at -20 °C.

Références pour anticorps anti-LYVE1 (ABIN115688)

Rangel-Moreno, de la Luz Garcia-Hernandez, Ramos-Payan, Biear, Hernady, Sangster, Randall, Johnston, Finkelstein, Williams: "Long-Lasting Impact of Neonatal Exposure to Total Body Gamma Radiation on Secondary Lymphoid Organ Structure and Function." dans: Radiation research, Vol. 184, Issue 4, pp. 352-66, (2015) (PubMed).

Heishi, Hosaka, Suzuki, Miyashita, Oike, Takahashi, Nakamura, Arioka, Mitsuda, Takakura, Hojo, Matsumoto, Yamauchi, Ohta, Sonoda, Sato: "Endogenous angiogenesis inhibitor vasohibin1 exhibits broad-spectrum antilymphangiogenic activity and suppresses lymph node metastasis." dans: The American journal of pathology, Vol. 176, Issue 4, pp. 1950-8, (2010) (PubMed).

Conrad, Niess, Huss, Huber, von Luettichau, Nelson, Ott, Jauch, Bruns: "Multipotent mesenchymal stem cells acquire a lymphendothelial phenotype and enhance lymphatic regeneration in vivo." dans: Circulation, Vol. 119, Issue 2, pp. 281-9, (2009) (PubMed).

Détails sur LYVE1

Antigène: LYVE1 (Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE1))

Autre désignation: LYVE-1 (LYVE1 Produits)

Synonymes: anticorps LYVE1, anticorps XLKD1, anticorps CRSBP-1, anticorps HAR, anticorps LYVE-1, anticorps 1200012G08Rik, anticorps Crsbp-1, anticorps Lyve-1, anticorps Xlkd1, anticorps lymphatic vessel endothelial hyaluronic receptor 1a, anticorps lymphatic vessel endothelial hyaluronan receptor 1, anticorps lyve1a, anticorps LYVE1, anticorps Lyve1

Sujet: LYVE-1 has been identified as a major receptor for HA (extracellular matrix glycosaminoglycan hyaluronan) on the lymph vessel wall. The deduced amino acid sequence of LYVE-1 predicts a 322-residue type I integral membrane polypeptide 41 % similar to the CD44 HA receptor with a 212- esidue extracellular domain containing a single Link module the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE-1 Molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE- molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE-1 is the first lymphspecific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves.Synonyms: CRSBP-1, CRSBP1, Cell surface retention sequence-binding protein 1, Extracellular link domain-containing protein 1, HAR, Hyaluronic acid receptor, LYVE1, Lymphatic vessel endothelial hyaluronic acid receptor 1, XLKD1

ID gène: 114332

NCBI Accession: NP_444477

UniProt: Q8BHC0

Pathways: Glycosaminoglycan Metabolic Process