CCL2 anticorps

N° du produit ABIN115767

Cité dans 1 publication. Cet anticorps anti-CCL2 Monoclonal Souris (ABIN115767) détecte spécifiquement CCL2 dans WB, IHC (p), EIA et IHC (fro). L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour CCL2 anticorps (ABIN115767)

Antigène: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CCL2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-CCL2 anticorps

Purification: Affinity Chromatography on Protein A

Immunogène: Highly pure (> 98%) E.Coli derived Recombinant Human MCP-1/MCAF (Cat.-No PA098)

Isotype: IgG1

Information d'application

Indications d'application: ELISA: In a Sandwich ELISA (assuming 100 μL/well), a concentration of 2-4 μg/mL of thisantibody will detect at least 300 pg/mL of recombinant Human MCP1 when used withBiotinynaled affinity purified anti-Human MCP1 antibody (PP1044B1 or PP1044B2). Western Blot: To detect Human MCP1 by Western Blot analysis this antibody can be used ata concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant Human MCP1 is 1.0-2.0 ng/lane, underreducing or non-reducing conditions. Immunohistochemistry: 10 μg/mL with an Overnight Incubation at 4 °C. This MCP1 antibody stained Formalin-Fixed, Paraffin-Embedded Sections of Human breastinvasive ductal carcinoma. An HRP-labeled polymer detection system was used with a DABChromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer isrecommended.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with sterile water to a concentration of 1.0 mg/mL.

Buffer: PBS without preservatives

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Références pour anticorps anti-CCL2 (ABIN115767)

Betz, Blood, Blood: "University students' perceptions of pre-school and kindergarten children who stutter." dans: Journal of communication disorders, Vol. 41, Issue 3, pp. 259-73, (2008) (PubMed).

Détails sur CCL2

Antigène: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Autre désignation: MCP1 / CCL2

Sujet: This chemotactic factor attracts monocytes and basophils but not neutrophils or eosinophils and augments monocyte anti-tumor activity. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. It may be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.Synonyms: C-C motif chemokine 2, HC-11, HC11, MCAF, MCP-1, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, SCYA2, Small-inducible cytokine A2

ID gène: 9606

UniProt: P13500

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, Phosphorylation & l'infection par le SRAS-CoV-2