KIT Ligand anticorps

N° du produit ABIN115909

Cet anticorps Lapin Polyclonal détecte spécifiquement KIT Ligand dans WB, EIA et Func. Il présente une réactivité envers Souris.

Aperçu rapide pour KIT Ligand anticorps (ABIN115909)

Antigène: KIT Ligand (KITLG)

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp KIT Ligand est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Détail du produit anti-KIT Ligand anticorps

Specificité: This antibody recognises Murine Stem Cell Factor.

Purification: Affinity chromatography.

Immunogène: Highly pure (>98%) recombinant mSCF.

Information d'application

Indications d'application: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mSCF (25.0 ng/mL),a concentration of 0.60-0.65 μg/mL of this antibody is required. ELISA: To detect mSCF by direct ELISA (using 100 l/well antibody solution) a concentration of atleast 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, inconjunction with compatible secondary reagents, allows the detection of 0.2-0.4 ng/wellof recombinant mSCF. Western Blot: To detect mSCF by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant mSCF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Détails sur KIT Ligand

Antigène: KIT Ligand (KITLG)

Autre désignation: KITLG / SCF

Sujet: Synonyms: KITL, Kit ligand, MGF, Mast cell growth factor, Stem cell factor, c-Kit ligand

ID gène: 17311

NCBI Accession: NP_038626

UniProt: P20826

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway