FGF1 anticorps
Aperçu rapide pour FGF1 anticorps (ABIN116005)
Antigène
Voir toutes FGF1 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Specificité
- Human Fibroblast Growth Factor acidic (hFGF-1)
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Purification
- Affinity chromatography.
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Immunogène
- Highly pure (>98%) recombinant hFGF-acidic.
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Indications d'application
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Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhFGF-acidic (30 ng/mL), a concentration of 0.46 - 0.9 μg/mL of this antibody is required. ELISA: To detect hFGF-acidic by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2 - 0.4 ng/well of recombinant hFGF-acidic. Western Blot: To detect hFGF-acidic by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hFGF-acidic is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Reconstitution
- Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
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Buffer
- PBS, pH 7.2 without preservatives.
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Agent conservateur
- Without preservative
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Conseil sur la manipulation
- Centrifuge vial prior to opening!
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Stock
- 4 °C
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- FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))
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Autre désignation
- FGF Acidic / FGF1
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Sujet
- The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these two growth factors.Synonyms: Acidic fibroblast growth factor, Beta-endothelial cell growth factor, ECGF-beta, FGFA, HBGF-1, HBGF1, Heparin-binding growth factor 1
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ID gène
- 2246
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NCBI Accession
- NP_000791
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UniProt
- P05230
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Pathways
- Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Antigène
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