Interferon gamma anticorps

N° du produit ABIN116053

Cet anticorps Lapin Polyclonal détecte spécifiquement Interferon gamma dans WB, IHC (p), Func et EIA. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour Interferon gamma anticorps (ABIN116053)

Antigène: Interferon gamma (IFNG)

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Interferon gamma est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func), Enzyme Immunoassay (EIA)

Détail du produit anti-Interferon gamma anticorps

Purification: Affinity Chromatography using immobilized Human IFN-gamma matrix

Immunogène: Highly purified (>98%) E.coli derived recombinant Human Inferferon gamma (Cat.-No PA070)

Information d'application

Indications d'application: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofh-IFN-gamma (50 pg/mL), a concentration of 0.04-0.06 μg/mL of the previous lot of thisantibody was required. ELISA: To detect h-IFN-gamma by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2-0.4 ng/well of recombinant h-IFN-gamma. Western blot: To detect h-IFN-gamma by Western Blot analysis this antibody can be used ata concentration of 0.1- 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant h-IFN-gamma is 1.5-3.0 ng/lane, under either reducingor non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Détails sur Interferon gamma

Antigène: Interferon gamma (IFNG)

Autre désignation: IFNG / Interferon gamma

Sujet: Interferon gamma is mainly produced by T lymphocytes and NK cells. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses,including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak antiviral and antiproliferative activity, and poteniates the antiviral and anti tumor effects of IFN alpha / beta (type I interferon). It is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. Labile at pH 2.Synonyms: IFN-gamma, gamma IFN

ID gène: 9606

UniProt: P01579

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy