IGF2 anticorps

N° du produit ABIN116059

Cet anticorps Lapin Polyclonal détecte spécifiquement IGF2 dans WB, IHC (p), Func et EIA. Il présente une réactivité envers Humain.

Aperçu rapide pour IGF2 anticorps (ABIN116059)

Antigène: IGF2 (Insulin-Like Growth Factor 2 (IGF2))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IGF2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func), Enzyme Immunoassay (EIA)

Détail du produit anti-IGF2 anticorps

Purification: Affinity chromatography employing immobilized hhIGF-II matrix

Immunogène: Highly pure (>98%) recombinant human IGF-II.

Information d'application

Indications d'application: NeutralizationTo yield one-half maximal inhibition [ND50] of the biological activity of hIGF-II (10 ng/mL), aconcentration of 3.1 - 4.6 μg/mL of this antibody is required. ElisaIndirect: To detect hIGF-II by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-II. Sandwich: To detect hIGF-II by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL is required. In conjunction with Biotinylated Anti-HumanIGF-II as a detection antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hIGF-II. Western BlotTo detect hIGF-II by Western Blot analysis this antibody can be used at a concentration of0.1- 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant hIGF-II is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.

Restrictions: For Research Use only

Stockage

Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2

Conseil sur la manipulation: This product is photosensitive and should be protected from light. Should it contain a precipitate we recommend microcentrifugation before use.

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

Détails sur IGF2

Antigène: IGF2 (Insulin-Like Growth Factor 2 (IGF2))

Autre désignation: Insulin-Like Growth Factor II / IGF2

Sujet: The IGF2 gene encodes a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is expressed only from the paternally inherited allele and is an imprinted gene which is a candidate gene for eating disorders. There is a read-through, INS-IGF2, which aligns to this gene at the 3' region and to the upstream INS gene at the 5' region. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. Insulin Like Growth Factor-2 (IGF-2) is a polypeptide growth factor, which stimulates the proliferation of a wide range of cell types.Synonyms: IGF-II, Somatomedin-A

ID gène: 3481

NCBI Accession: NP_000603

UniProt: P01344

Pathways: Hormone Activity, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Activated T Cell Proliferation