IL-3 anticorps

N° du produit ABIN116071

Détail du produit anti-IL-3 anticorps

Antigène: IL-3 (Interleukin 3 (IL-3))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IL-3 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Specificité: Specific for human IL-3.

Purification: Affinity chromatography.

Immunogène: Highly pure (>98%) recombinant hIL-3.

Information d'application

Indications d'application: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-3(3.0 ng/mL), a concentration of 0.06 - 0.1 μg/mL of this antibody is required. ELISA: To detect hIL-3 by direct ELISA (using 100 l/well antibody solution) a concentrationof at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, inconjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/wellof recombinant hIL-3. Western Blot: To detect hIL-3 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hIL-3 is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Détails sur IL-3

Antigène: IL-3 (Interleukin 3 (IL-3))

Autre désignation: Interleukin-3 / IL3 (IL-3 Produits)

Synonymes: anticorps BPA, anticorps Csfmu, anticorps HCGF, anticorps Il-3, anticorps MCGF, anticorps PSF, anticorps IL-3, anticorps MULTI-CSF, anticorps interleukin 3, anticorps interleukin 3 (colony-stimulating factor, multiple), anticorps Il3, anticorps IL3

Sujet: The pleiotropic IL3 (Interleukin 3) is a 15 kDa cytokine that is primarily secreted by activated T lymphocytes and stimulates the proliferation and differentiation of hematopoietic cells. IL3 acts in hematopoiesis by controlling the production, differentiation, and function of two related white cell populations of the blood, the granulocytes and the monocytes/macrophages. It induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes and is expressed by activated T cells, mast cells, and natural killer cells. IL3 not only supports growth of both pluripotent stem cells and the more differentiated committed progenitors, but it also stimulates the functional activity of some fully differentiated cells. IL3 has also been shown to protect mast cells from undergoing apoptosis.Synonyms: Hematopoietic growth factor, IL-3, MCGF, Mast cell growth factor, Multipotential colony-stimulating factor, P-cell-stimulating factor

ID gène: 3562

NCBI Accession: NP_000579

UniProt: P08700

Pathways: Signalistation JAK/STAT, Regulation of Carbohydrate Metabolic Process, Autophagy