FGF7 anticorps

N° du produit ABIN116128

Détail du produit anti-FGF7 anticorps

Antigène: FGF7 (Fibroblast Growth Factor 7 (FGF7))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp FGF7 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)

Specificité: This antibody reacts with Human Keratinocyte Growth Factor (KGF).

Purification: Affinity chromatography

Immunogène: Highly purified (>98%) recombinant FGF-7 (KGF)

Information d'application

Indications d'application: Neutralisation: To yield one-half maximal inhibition [ND50]of the biological activity of hKGF(25 ng/mL), a concentration of 0.56-0.85 μg/mL of this antibody has been used for previouslots. ELISA: To detect hKGF by direct ELISA (using 100 l/well antibody solution) a concentrationof at least 0.5g/mL of this antibody is required. This antigen affinity purified antibody, inconjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/wellof recombinant hKGF. Western blot: To detect hKGF by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hKGF is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Références pour anticorps anti-FGF7 (ABIN116128)

Lim, Poh, Riau, Beuerman, Tan, Mehta: "Biological and ultrastructural properties of acelagraft, a freeze-dried γ-irradiated human amniotic membrane." dans: Archives of ophthalmology, Vol. 128, Issue 10, pp. 1303-10, (2010) (PubMed).

Détails sur FGF7

Antigène: FGF7 (Fibroblast Growth Factor 7 (FGF7))

Autre désignation: FGF7 / KGF (FGF7 Produits)

Synonymes: anticorps HBGF-7, anticorps KGF, anticorps Kgf, anticorps kgf, anticorps FGF-7, anticorps fgf7, anticorps fibroblast growth factor 7, anticorps fibroblast growth factor 7 L homeolog, anticorps FGF7, anticorps Fgf7, anticorps fgf7, anticorps fgf7.L

Sujet: KGF is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis.Synonyms: Fibroblast growth factor 7, HBGF7, Heparin-binding growth factor 7, Keratinocyte growth factor

ID gène: 2252

NCBI Accession: NP_002000

UniProt: P21781

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway