KIT Ligand anticorps (Biotin)
Aperçu rapide pour KIT Ligand anticorps (Biotin) (ABIN116223)
Antigène
Voir toutes KIT Ligand (KITLG) AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Specificité
- Human Stem Cell Factor (hSCF).
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Purification
- Affinity chromatography.
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Immunogène
- Highly pure (>98%) recombinant hSCF.
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Indications d'application
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ELISA: To detect hSCF by direct ELISA (using 100 l/well antibody solution) this antibodycan be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant hSCF. Western Blot: To detect hSCF by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 mg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hSCF is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Reconstitution
- Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
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Buffer
- PBS, pH 7.2 without preservatives.
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Agent conservateur
- Without preservative
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Conseil sur la manipulation
- Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
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Stock
- 4 °C/-20 °C
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Stockage commentaire
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- KIT Ligand (KITLG)
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Autre désignation
- KITLG / SCF
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Sujet
- Synonyms: KITL, Kit ligand, MGF, Mast cell growth factor, Stem cell factor, c-Kit ligand
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ID gène
- 4254
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NCBI Accession
- NP_000890
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UniProt
- P21583
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Pathways
- Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Antigène
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