TGFA anticorps

N° du produit ABIN116239

Cet anticorps Lapin Polyclonal détecte spécifiquement TGFA dans WB, IHC (p), EIA et Func. Il présente une réactivité envers Humain.

Aperçu rapide pour TGFA anticorps (ABIN116239)

Antigène: TGFA (Transforming Growth Factor, alpha (TGFA))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TGFA est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)

Détail du produit anti-TGFA anticorps

Specificité: This antibody reacts with Transforming Growth Factor alpha.

Purification: Affinity chromatography.

Immunogène: Highly pure (>98%) recombinant hTGF-alpha

Information d'application

Indications d'application: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhTGF-α (0.5 ng/mL), a concentration of 2.5-2.7 μg/mL of this antibody is required. ELISA: (Indirect): To detect hTGF-α by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hTGF-α. (Sandwich): To detect hTGF-α by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Human TGF-α (PP1070B1 or PP1070B2) as adetection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTGF-α. Western Blot: To detect hTGF-α by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hTGF-α is 1.5- 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Détails sur TGFA

Antigène: TGFA (Transforming Growth Factor, alpha (TGFA))

Autre désignation: TGF-alpha (TGFA)

Sujet: Synonyms: EGF-like TGF, ETGF, TGF type 1, Transforming growth factor alpha

ID gène: 7039

NCBI Accession: NP_001093161

UniProt: P01135

Pathways: Signalisation NF-kappaB, Signalisation RTK, EGFR Signaling Pathway