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Midkine anticorps (Biotin)

Cet anticorps anti-Midkine est un anticorps Lapin Polyclonal détectant Midkine dans WB et EIA. Adapté pour Humain.
N° du produit ABIN116439

Aperçu rapide pour Midkine anticorps (Biotin) (ABIN116439)

Antigène

Voir toutes Midkine (MDK) Anticorps
Midkine (MDK) (Midkine (Neurite Growth-Promoting Factor 2) (MDK))

Reactivité

  • 80
  • 35
  • 33
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Humain

Hôte

  • 79
  • 14
Lapin

Clonalité

  • 71
  • 22
Polyclonal

Conjugué

  • 41
  • 17
  • 8
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp Midkine est conjugé à/à la Biotin

Application

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  • 1
Western Blotting (WB), Enzyme Immunoassay (EIA)
  • Purification

    Affinity chromatography.

    Immunogène

    Highly pure (>98%) recombinant hMidkine.
  • Indications d'application

    ELISA: To detect hMidkine by direct ELISA (using 100 l/well antibody solution) thisantibody can be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction withcompatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinanthMidkine. Western Blot: To detect hMidkine by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hMidkine is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Reconstitution

    Restore to a concentration of 50 μg/mL with sterile PBS solution containing 0.1 % BSA.

    Buffer

    PBS, pH 7.2 without preservatives.

    Agent conservateur

    Without preservative

    Conseil sur la manipulation

    Avoid repeated freezing and thawing.

    Stock

    4 °C/-20 °C

    Stockage commentaire

    Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
  • Antigène

    Midkine (MDK) (Midkine (Neurite Growth-Promoting Factor 2) (MDK))

    Autre désignation

    Midkine

    Sujet

    Midkine exhibits neurite outgrowth-promoting activity and may play a role in nervous system development and/or maintenance. Its expression is predominant only for a short period from approximately one-half to two-thirds of the way through gestation, before and after that, it is barely detectable. Midkine was first found in differentiating mouse teratocarcinoma cells. It has neurotrophic activities and is mitogenic to certain, but not to all, fibroblast cell lines.Synonyms: Amphiregulin-associated protein, MDK, MK, MK1, Midgestation and kidney protein, NEGF2, Neurite outgrowth-promoting factor 2, Neurite outgrowth-promoting protein

    ID gène

    4192

    NCBI Accession

    NP_001012333

    UniProt

    P21741

    Pathways

    Signalisation RTK, M Phase, Skeletal Muscle Fiber Development
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