CDKN1B anticorps

N° du produit ABIN116519

L’anticorps anti-CDKN1B Polyclonal Lapin est utilisé pour la détection de CDKN1B dans des échantillons de Humain. Il a été validé pour WB, IP et EIA.

Aperçu rapide pour CDKN1B anticorps (ABIN116519)

Antigène: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CDKN1B est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Détail du produit anti-CDKN1B anticorps

Specificité: This product was Antiserum will specifically react with a p27 protein from human tissue. No reaction was observed against other related tumor suppressor proteins. No reactivity is observed with p27 from mouse. Reactivity against p27 from other species is unknown.

Purification: Prepared from monospecific antiserum by delipidation and defibrination.

Immunogène: Full length human p27 protein.

Information d'application

Indications d'application: Suitable for ELISA, Immunoprecipitation, Immunoblotting and other immunologicalmethods requiring high titer and specificity. Recommended Dilutions: This product was assayed by immunoblot and found to bereactive against p27 from cell lysates and E. coli produced fusion proteins at a dilution of1: 2000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG[H&L] (Goat). Expect a band at approx. 27 kDa in size corresponding to p27 by western blotting inthe appropriate cell lysate or extract. Anti-p27 is suitable for the detection by immunoblot of human, rat and mouse p27. This antisera is suitable for Immmunoprecipitation of in vitro translated protein and celllysates (C212, 3T3-L1) and will co-precipitate associated proteins.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 80 mg/mL (by Refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store original vial and reconstituted antibody at 2-8 °C.

Détails sur CDKN1B

Antigène: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Autre désignation: CDKN1B / KIP1

Sujet: P27 Kip is a cyclin-dependent kinase inhibitor that shares a limited similarity with CDK inhibitor CDKN1A/p21. p27 binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.Synonyms: Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1

ID gène: 1027

NCBI Accession: NP_004055

UniProt: P46527

Pathways: Cycle Cellulaire, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy