Recombinant Golgin B1 (GOLGB1) anticorps

N° du produit ABIN1169059

Cet anticorps Human Monoclonal détecte spécifiquement Golgin B1 dans ICC. Il présente une réactivité avec des échantillons de Humain et Souris. Il a été cité dans 2+ publications.

Aperçu rapide pour Recombinant Golgin B1 (GOLGB1) anticorps (ABIN1169059)

Antigène: Golgin B1 (GOLGB1)

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Souris

Hôte: Human

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Immunocytochemistry (ICC)

Clone: TA10

Détail du produit

Fonction: anti-Giantin, mAb (rec.) (TA10)

Specificité: Recognizes human and mouse giantin.

Attributs du produit: Recombinant Antibody. Recognizes human and mouse giantin. Species cross-reactivity: Human, Mouse. Clone: TA10. Isotype: Human IgG2lambda. Applications: ICC. Host: Purified from HEK 293 cell culture supernatant. Liquid. In PBS containing 10 % glycerol and 0.02 % sodium azide. Giantin, the largest Golgi membrane protein, is C-terminally anchored to cis- and medial-Golgi membranes and forms a long ~250nm coiled-coil rod that directly interacts with microtubules in a cell-cycle regulated way. It is an excellent marker for mammalian golgi complex. It is also an antigen in chronic rheumatoid arthritis and in the autoimmune disease Sjoegren syndrome.

Purification: Purified from HEK 293 cell culture supernatant.

Pureté: >95 % (SDS-PAGE)

Immunogène: Human recombinant giantin.

Isotype: IgG2 lambda

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

anti-Giantin, monoclonal antibody (recombinant) (TA10) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain. anti-Giantin, monoclonal antibody (recombinant) (TA10) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: In PBS containing 10 % glycerol and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: After opening, prepare aliquots and store at -20 °C.Avoid freeze/thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire:

+4°C

-20°C

Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.

Date de péremption: 12 months

Référence

Vielemeyer, Nizak, Jimenez, Echard, Goud, Camonis, Rain, Perez: "Characterization of single chain antibody targets through yeast two hybrid." dans: BMC biotechnology, Vol. 10, pp. 59, (2010) (PubMed).

Nizak, Martin-Lluesma, Moutel, Roux, Kreis, Goud, Perez: "Recombinant antibodies against subcellular fractions used to track endogenous Golgi protein dynamics in vivo." dans: Traffic (Copenhagen, Denmark), Vol. 4, Issue 11, pp. 739-53, (2003) (PubMed).

Détail du antigène

Antigène: Golgin B1 (GOLGB1)

Autre désignation: Giantin

Sujet:

Alternate Names/Synonyms: Golgin Subfamily B Member 1, 372 kDa Golgi Complex-associated Protein, GCP372, Macrogolgin

Product Description: Giantin, the largest Golgi membrane protein, is C-terminally anchored to cis- and medial-Golgi membranes and forms a long ~250nm coiled-coil rod that directly interacts with microtubules in a cell-cycle regulated way. It is an excellent marker for mammalian golgi complex. It is also an antigen in chronic rheumatoid arthritis and in the autoimmune disease Sjoegren syndrome.

UniProt: Q14789

Pathways: SARS-CoV-2 Protein Interactome