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CD25 anticorps (AA 24-225) (Biotin)

L’anticorps Lapin Polyclonal anti-CD25 a été validé pour WB, IHC et ICC. Il convient pour détecter CD25 dans des échantillons de Rat.
N° du produit ABIN1175093

Aperçu rapide pour CD25 anticorps (AA 24-225) (Biotin) (ABIN1175093)

Antigène

Voir toutes CD25 (IL2RA) Anticorps
CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reactivité

  • 229
  • 141
  • 42
  • 11
  • 10
  • 5
  • 3
  • 3
  • 3
  • 2
  • 1
  • 1
Rat

Hôte

  • 189
  • 104
  • 94
  • 3
  • 2
  • 2
  • 2
  • 1
Lapin

Clonalité

  • 320
  • 72
  • 4
Polyclonal

Conjugué

  • 143
  • 39
  • 30
  • 30
  • 24
  • 11
  • 8
  • 7
  • 5
  • 5
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp CD25 est conjugé à/à la Biotin

Application

  • 265
  • 95
  • 87
  • 59
  • 56
  • 55
  • 31
  • 25
  • 16
  • 16
  • 10
  • 9
  • 9
  • 8
  • 8
  • 6
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
  • Épitope

    • 23
    • 16
    • 7
    • 6
    • 6
    • 6
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 24-225

    Fonction

    Biotin-Linked Polyclonal Antibody to Interleukin 2 Receptor Alpha (IL2Ra)

    Séquence

    MGHHHHHHSG SEF-CLYDPPE VPNATFKALS YKNGTILNCE CKRGFRRLNE LVYMACLGNS WSNNCQCTSN SHDNSREQVT PQPEGQKEQQ TTDTQKSTQS VYQENLAGHC REPPPWRHED TKRIYHFVEG QIVLYTCIQG YKALQRGPAI SICKTVCGEI RWTHPQLTCV DEKEHHQFLA SEESQGSRNS FPESEASCPT PNTDFSQLTE ATTTM

    Specificité

    The antibody is a rabbit polyclonal antibody raised against IL2Ra. It has been selected for its ability to recognize IL2Ra in immunohistochemical staining and western blotting.

    Purification

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography

    Immunogène

    Recombinant IL2Ra expressed in E.coli.
    The antibody is a rabbit polyclonal antibody raised against IL2Ra conjugated to biotin.

    Isotype

    IgG
  • Indications d'application

    Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

    Commentaires

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    500 μg/mL

    Buffer

    PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

    Conseil sur la manipulation

    Avoid repeated freeze/thaw cycles

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

    Date de péremption

    12 months
  • Antigène

    CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

    Autre désignation

    Interleukin 2 Receptor Alpha

    Sujet

    CD25, IL2R-a, IL2-RA, IDDM10, IL2R, TCGFR, p55, TAC antigen

    Pathways

    Signalistation JAK/STAT, Growth Factor Binding, Activated T Cell Proliferation
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