NGFR anticorps

N° du produit ABIN1176837

Cet anticorps anti-NGFR est un anticorps Souris Monoclonal détectant NGFR dans WB, FACS, IHC (p), IP, ICC, ICFC et MS. Adapté pour Humain, Non-Human Primate, Lapin, Cat et Furet. Ce Primary Antibody a été cité dans 4+ publications.

Aperçu rapide pour NGFR anticorps (ABIN1176837)

Antigène: NGFR (Nerve Growth Factor Receptor (NGFR))

Reactivité: Humain, Non-Human Primate, Lapin, Cat, Furet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp NGFR est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC), Intracellular Flow Cytometry (ICFC), Mass Spectrometry (MS)

Clone: NGFR5

Détail du produit anti-NGFR anticorps

Fonction: Anti-Hu CD271 Purified

Specificité: The mouse monoclonal antibody NGFR5 (originally C34C) recognizes an epitope within ammino acids 1 - 160 of CD271/NGFR, a 75 kDa transmembrane glycoprotein of the TNFR superfamily.

Aucune reactivité croisée: Souris, Rat

Réactivité croisée (Details): Human, Non-Human Primates, Feline (Cat), Rabbit, Ferret

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Purified CD271 protein isolated from human melanoma cell line A875

Isotype: IgG1

Information d'application

Indications d'application: Immunohistochemistry (paraffin sections): Recommended dilution: 1-2 μg/mL, Positive tissue: melanoma, heat-mediated antigen retrieval.
Flow cytometry: Extracellular and intracellular staining, recommended dilution: 1-4 μg/mL.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-NGFR (ABIN1176837)

Alpers, Hudkins, Ferguson, Johnson, Schatteman, Bothwell: "Nerve growth factor receptor expression in fetal, mature, and diseased human kidneys." dans: Laboratory investigation; a journal of technical methods and pathology, Vol. 69, Issue 6, pp. 703-13, (1994) (PubMed).

Thompson, Schatteman, Gown, Bothwell: "A monoclonal antibody against nerve growth factor receptor. Immunohistochemical analysis of normal and neoplastic human tissue." dans: American journal of clinical pathology, Vol. 92, Issue 4, pp. 415-23, (1989) (PubMed).

Schatteman, Gibbs, Lanahan, Claude, Bothwell: "Expression of NGF receptor in the developing and adult primate central nervous system." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 8, Issue 3, pp. 860-73, (1988) (PubMed).

Marano, Dietzschold, Earley, Schatteman, Thompson, Grob, Ross, Bothwell, Atkinson, Koprowski: "Purification and amino terminal sequencing of human melanoma nerve growth factor receptor." dans: Journal of neurochemistry, Vol. 48, Issue 1, pp. 225-32, (1987) (PubMed).

Détails sur NGFR

Antigène: NGFR (Nerve Growth Factor Receptor (NGFR))

Autre désignation: CD271

Sujet: Nerve growth factor receptor,CD271 / NGFR, also known as p75NGFR or p75NTR, is a 75 kDa low affinity receptor for the NGF (nerve growth factor), BDNF (brain-derived growth factor), and other neurotrophins, such as NT3 and NT4/5. Unlike other members of the tumor necrosis factor receptor superfamily of transmembrane proteins, CD271 has unique intracellular domain structure (lacks catalytic activity) and downstream signaling partners. Triggered by its ligands CD271 affects growth, differentiation, migration and death of the nervous system cells.,TNFRSF16, NGFR, p75NTR, p75NGFR

ID gène: 4804

UniProt: P08138

Pathways: Signalisation NF-kappaB, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding