IL-2 anticorps

N° du produit ABIN1176901

Détail du produit anti-IL-2 anticorps

Antigène: IL-2 (IL2) (Interleukin 2 (IL2))

Reactivité: Humain

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp IL-2 est non-conjugé

Application: ELISA, Neutralization (Neut), Intracellular Staining (ICS)

Marque: BD Pharmingen™

Attributs du produit: Neutralization Activity: This antibody has been reported to be useful for the neutralization of recombinant human IL-2. Neutralization activity may be measured with a proliferation assay using 100 pg/mL recombinant human IL-2 (Cat. No. 554603) to stimulate CTLL-2 cells at 2x10^4 cells/mL as indicator cells (i.e. preincubation of the antibody with recombinant human IL-2 for 60 minutes).
50% Neutralization (ND50) at 0.1-1.5 µg/mL
>95% Neutralization at 3 - 15 myg/mL

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogène: Human IL-2 Recombinant Protein

Clone: MQ1-17H12

Isotype: IgG2a kappa

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Agent conservateur: Azide free

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Références pour anticorps anti-IL-2 (ABIN1176901)

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." dans: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).

Andersson, Abrams, Björk, Funa, Litton, Agren, Andersson: "Concomitant in vivo production of 19 different cytokines in human tonsils." dans: Immunology, Vol. 83, Issue 1, pp. 16-24, (1995) (PubMed).

Fernandez, Andersson, Andersson, Troye-Blomberg: "Cytokine synthesis analyzed at the single-cell level before and after revaccination with tetanus toxoid." dans: European journal of immunology, Vol. 24, Issue 8, pp. 1808-15, (1994) (PubMed).

Gillis, Ferm, Ou, Smith: "T cell growth factor: parameters of production and a quantitative microassay for activity." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 120, Issue 6, pp. 2027-32, (1978) (PubMed).

Détails sur IL-2

Antigène: IL-2 (IL2) (Interleukin 2 (IL2))

Autre désignation: IL-2 (IL2 Produits)

Synonymes: anticorps IL2, anticorps IL-2, anticorps il2, anticorps TCGF, anticorps lymphokine, anticorps Il-2, anticorps POIL2, anticorps interleukin 2, anticorps interleukin-15, anticorps IL2, anticorps il2, anticorps LOC100303688, anticorps Il2

Sujet: The MQ1-17H12 antibody reacts with human interleukin-2 (IL-2). The immunogen used to generate the MQ1-17H12 hybridoma was recombinant human IL-2. Unconjugated or purified forms of this antibody have been reported to be neutralizing for human IL-2 bioactivity.

Pathways: Signalistation JAK/STAT, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation