IL3RA anticorps

N° du produit ABIN1176902

L’anticorps Souris Monoclonal anti-IL3RA a été validé pour FACS et Neut. Il convient pour détecter IL3RA dans des échantillons de Humain. Il y a 1 publication disponible.

Aperçu rapide pour IL3RA anticorps (ABIN1176902)

Antigène: IL3RA (Interleukin 3 Receptor, alpha (IL3RA))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp IL3RA est non-conjugé

Application: Flow Cytometry (FACS), Neutralization (Neut)

Clone: 7G3

Détail du produit anti-IL3RA anticorps

Marque: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogène: Human IL-3Ra-transfected cells

Isotype: IgG2a kappa

Information d'application

Indications d'application: This antibody has been found to block the binding of 125I-IL-3 to high and low affinity IL-3 receptors and can neutralize IL-3 bioactivity. In a TF-1 cell proliferation assay, this antibody (at ~0.5 µg/ml) was found to inhibit by 50% the proliferation induced by 0.3 ng/ml (~1 U/ml) of human IL-3. In functional experiments, the 7G3 antibody was found to inhibit acute myeloid leukemia cell proliferation, basophil histamine release, endothelial cell-mediated IL-8 secretion, and neutrophil transmigration. In various formats, i.e., purified with azide (Cat. No. 554527), biotinylated (Cat. No. 554528), and phycoerythrin-labeled (Cat. No. 554529), the 7G3 antibody is useful for applications such as immunoprecipitation, Western blotting, and immunofluorescent labeling of cells for flow cytometric analysis.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Agent conservateur: Azide free

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Références pour anticorps anti-IL3RA (ABIN1176902)

Sun, Woodcock, Rapoport, Stomski, Korpelainen, Bagley, Goodall, Smith, Gamble, Vadas, Lopez: "Monoclonal antibody 7G3 recognizes the N-terminal domain of the human interleukin-3 (IL-3) receptor alpha-chain and functions as a specific IL-3 receptor antagonist." dans: Blood, Vol. 87, Issue 1, pp. 83-92, (1996) (PubMed).

Détails sur IL3RA

Antigène: IL3RA (Interleukin 3 Receptor, alpha (IL3RA))

Autre désignation: CD123

Sujet: Reacts with human CD123, the 70 kD IL-3 receptor alpha chain (IL-3Ralpha), which associates with the 120-140 kD beta subunit. The beta chain is shared with the receptors for interleukins IL-5 and GM-CSF. IL-3Ralpha is expressed on hematopoietic progenitors and plays an important role in hematopoietic progenitor cell growth and differentiation. This antibody has been reported to block the binding of 125I-IL-3 to high and low affinity IL-3 receptors. In functional experiments, this antibody was found to inhibit acute myeloid leukemia cell proliferation, basophil histamine release, endothelial cell-mediated IL-8 secretion, and neutrophil transmigration. This antibody has been reported to be useful for immunoprecipitation, western blot and immunofluorescent staining for flow cytometry. At the Fifth HLDA Workshop, the human IL-3 receptor was designated CD123. This antibody is routinely tested by LAL for endotoxin level and by immunofluorescent staining and flow cytometry.
Synonyms: IL-3 Receptor alpha chain

Pathways: Signalistation JAK/STAT