IRF7 anticorps (PE)

N° du produit ABIN1176906

Cet anticorps anti-IRF7 est un anticorps Souris Monoclonal détectant IRF7 dans ICS. Adapté pour Humain. Ce Primary Antibody a été cité dans 1 publication.

Aperçu rapide pour IRF7 anticorps (PE) (ABIN1176906)

Antigène: IRF7 (Interferon Regulatory Factor 7 (IRF7))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp IRF7 est conjugé à/à la PE

Application: Intracellular Staining (ICS)

Clone: K40-321

Détail du produit anti-IRF7 anticorps (PE)

Marque: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human IRF-7 Recombinant Protein

Isotype: IgG1 kappa

Information d'application

Commentaires:

BALB/c

Volume d'échantillon: 20 μL

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-IRF7 (PE) (ABIN1176906)

Sharma, tenOever, Grandvaux, Zhou, Lin, Hiscott: "Triggering the interferon antiviral response through an IKK-related pathway." dans: Science (New York, N.Y.), Vol. 300, Issue 5622, pp. 1148-51, (2003) (PubMed).

Détails sur IRF7

Antigène: IRF7 (Interferon Regulatory Factor 7 (IRF7))

Autre désignation: IRF-7

Sujet: Interferon regulatory factor 7 (IRF-7) is a transcription factor that regulates anti-viral defenses by controlling the induction of type-I interferon (IFN) responses. IRF-7 expression is induced in lymphoid cells by virus infection, as well as by IFN, lipopolysaccharide, and TNF-a. IRF-7 responses are initiated by Toll-like receptors (TLR) or the cytoplasmic protein retinoic acid inducible gene I (RIG-I). Upon TLR activation, it forms cytoplasmic complexes with MyD88, an adaptor in the TLR signaling pathways. The TLR-dependent and RIG-I-dependent pathways activate kinases, such as IKK-e and TBK1, that phosphorylate IRF-7 and induce movement of IRF-7-containing complexes to the nucleus, where it preferentially activates IFN-a promoters. The K40-321 monoclonal antibody recognizes human IRF-7, regardless of phosphorylation status. Our in-house testing is performed on a cell line that has been co-transfected with TBK1 and IRF-7. Phosphorylation of IRF-7 in the transfectants requires virus infection or over-expression of a signaling molecule of the RIG-I pathway, such as TBK1. Phosphorylation of endogenous IRF-7 in untransfected cells has not yet been detected. We confirmed that mAb K40-321 does not cross-react with TBK1 by Western blot analysis using the purified antibody.

Pathways: Signalisation TLR, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Autophagy