CD3 anticorps

N° du produit ABIN1177019

L’anticorps Souris Monoclonal anti-CD3 a été validé pour FACS, IP, IHC (fro), WB et BCA. Il convient pour détecter CD3 dans des échantillons de Rat. Il y a 4+ publications disponibles.

Aperçu rapide pour CD3 anticorps (ABIN1177019)

Antigène: CD3

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD3 est non-conjugé

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), Biochemical Assay (BCA)

Clone: G4-18

Détail du produit anti-CD3 anticorps

Marque: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogène: PHA-stimulated rat lymph node and spleen cells

Isotype: IgG3 kappa

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing protein stabilizer, no preservative, 0.2μm sterile filtered.

Agent conservateur: Azide free

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Références pour anticorps anti-CD3 (ABIN1177019)

Strickland, Kees, Holt: "Regulation of T-cell activation in the lung: alveolar macrophages induce reversible T-cell anergy in vitro associated with inhibition of interleukin-2 receptor signal transduction." dans: Immunology, Vol. 87, Issue 2, pp. 250-8, (1996) (PubMed).

Naper, Vaage, Lambracht, Løvik, Butcher, Wonigeit, Rolstad: "Alloreactive natural killer cells in the rat: complex genetics of major histocompatibility complex control." dans: European journal of immunology, Vol. 25, Issue 5, pp. 1249-56, (1995) (PubMed).

Upham, Strickland, Bilyk, Robinson, Holt: "Alveolar macrophages from humans and rodents selectively inhibit T-cell proliferation but permit T-cell activation and cytokine secretion." dans: Immunology, Vol. 84, Issue 1, pp. 142-7, (1995) (PubMed).

Nicolls, Aversa, Pearce, Spinelli, Berger, Gurley, Hall: "Induction of long-term specific tolerance to allografts in rats by therapy with an anti-CD3-like monoclonal antibody." dans: Transplantation, Vol. 55, Issue 3, pp. 459-68, (1993) (PubMed).

Détails sur CD3

Antigène: CD3

Autre désignation: CD3

Sujet: The G4.18 antibody reacts with the T-cell receptor-associated CD3 cell-surface antigen found on thymocytes, peripheral T lymphocytes, and dendritic epidermal T cells. It has been reported that CD3 expression is down-regulated within 24 hours in concanavalin A-stimulated rat T cells, and soluble mAb inhibits the allogeneic mixed-lymphocyte proliferative response and cell-mediated cytotoxicity to allogeneic target cells. In vivo treatment with G4.18 mAb prevents cardiac and skin allograft rejection, resulting in donor-specific tolerance. Pre-incubation of splenocytes with the alternate anti-rat CD3 monoclonal antibody, 1F4 (Cat. No. 556970), blocks staining with mAb G4.18.

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway