FAK anticorps (pSer910) (Alexa Fluor 647)

N° du produit ABIN1177061

L’anticorps Souris Monoclonal anti-FAK a été validé pour ICS. Il convient pour détecter FAK dans des échantillons de Humain, Souris et Rat. Il y a 3+ publications disponibles.

Aperçu rapide pour FAK anticorps (pSer910) (Alexa Fluor 647) (ABIN1177061)

Antigène: FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp FAK est conjugé à/à la Alexa Fluor 647

Application: Intracellular Staining (ICS)

Clone: K73-480

Détail du produit anti-FAK anticorps (Alexa Fluor 647)

Épitope: pSer910

Marque: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Phosphorylated Human FAK

Isotype: IgG2b kappa

Information d'application

Volume d'échantillon: 20 μL

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

Références pour anticorps anti-FAK (Alexa Fluor 647) (ABIN1177061)

Schlaepfer, Mitra, Ilic: "Control of motile and invasive cell phenotypes by focal adhesion kinase." dans: Biochimica et biophysica acta, Vol. 1692, Issue 2-3, pp. 77-102, (2004) (PubMed).

Ma, Richardson, Schaefer, Parsons: "Serine phosphorylation of focal adhesion kinase in interphase and mitosis: a possible role in modulating binding to p130(Cas)." dans: Molecular biology of the cell, Vol. 12, Issue 1, pp. 1-12, (2001) (PubMed).

Yamakita, Totsukawa, Yamashiro, Fry, Zhang, Hanks, Matsumura: "Dissociation of FAK/p130(CAS)/c-Src complex during mitosis: role of mitosis-specific serine phosphorylation of FAK." dans: The Journal of cell biology, Vol. 144, Issue 2, pp. 315-24, (1999) (PubMed).

Détails sur FAK

Antigène: FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))

Autre désignation: FAK

Sujet: Focal Adhesion Kinase (FAK) is a cytoplasmic tyrosine kinase that associates with integrins in focal adhesions. Its cellular localization is directed by a 125-amino acid sequence at the C terminus called the Focal Adhesion Targeting (FAT) domain, and the phosphorylation state of serine 910 (S910) in the FAT domain may regulate the assembly of focal adhesions. Furthermore, the binding of extracellular matrix ligands to integrins triggers tyrosine phosphorylations near FAK’s kinase domain that increase its kinase activity, and additional tyrosine phosphorylations near proline-rich motifs create binding sites for the SH2 domains of various adaptor proteins. FAK's ability to bind numerous structural and signaling proteins via a variety of interactions regulates FAK’s targeting to focal adhesions, modulates its kinase activity, and initiates intracellular signaling cascades. Thus, studies suggest that FAK may integrate cellular events controlling cell motility, growth, and invasiveness. The K73-480 monoclonal antibody recognizes the phosphorylated S910 of human FAK. The orthologous phosphorylation sites in mouse and rat FAK are S948 and S913, respectively.

Pathways: Response to Growth Hormone Stimulus, CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling