BCAR1 anticorps (pTyr249) (Alexa Fluor 647)

N° du produit ABIN1177115

Détail du produit anti-BCAR1 anticorps (Alexa Fluor 647)

Antigène: BCAR1 (Breast Cancer Anti-Estrogen Resistance 1 (BCAR1))

Épitope: pTyr249

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp BCAR1 est conjugé à/à la Alexa Fluor 647

Application: Intracellular Staining (ICS)

Marque: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Phosphorylated Human p130Cas

Clone: J169-757-12-2

Isotype: IgG2b kappa

Information d'application

Volume d'échantillon: 20 μL

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-BCAR1 (Alexa Fluor 647) (ABIN1177115)

Goldberg, Alexander, Pellicena, Zhang, Tsuda, Miller: "Src phosphorylates Cas on tyrosine 253 to promote migration of transformed cells." dans: The Journal of biological chemistry, Vol. 278, Issue 47, pp. 46533-40, (2003) (PubMed).

Hinsby, Olsen, Bennett, Mann: "Signaling initiated by overexpression of the fibroblast growth factor receptor-1 investigated by mass spectrometry." dans: Molecular & cellular proteomics : MCP, Vol. 2, Issue 1, pp. 29-36, (2003) (PubMed).

Détails sur BCAR1

Antigène: BCAR1 (Breast Cancer Anti-Estrogen Resistance 1 (BCAR1))

Autre désignation: p130 Cas (BCAR1 Produits)

Synonymes: anticorps CAS, anticorps CAS1, anticorps CASS1, anticorps CRKAS, anticorps P130Cas, anticorps AI385681, anticorps Cas, anticorps Crkas, anticorps P130CAS, anticorps MGC68466, anticorps cas, anticorps p130cas, anticorps BCAR1, anticorps fc17f11, anticorps wu:fc17f11, anticorps zgc:175192, anticorps BCAR1, Cas family scaffolding protein, anticorps breast cancer anti-estrogen resistance 1, anticorps breast cancer anti-estrogen resistance 1 L homeolog, anticorps breast cancer anti-estrogen resistance protein 1, anticorps BCAR1, anticorps Bcar1, anticorps bcar1.L, anticorps bcar1, anticorps LOC100435586

Sujet: P47v-crk (v-Crk) is the product of a transforming gene, v-crk, that was isolated from avian sarcoma viruses. The v-Crk protein is a fusion product of viral Gag protein and a part of cellular Crk that includes SH2 and SH3 domains. v-Crk-induced transformation increases tyrosine phosphorylation of several cellular proteins, including p130Cas (CRK-associated substrate). The p130Cas is tightly associated with v-Crk via the SH2 domain of v-Crk. Tyrosine phosphorylation of p130Cas occurs in conjunction with cellular transformation in cells that express v-Src or v-Crk. This phosphorylation leads to a change in p130Cas localization from the cytoplasm to the cell membrane and, possibly, to the nucleus. Since p130Cas also associates with v-Src, it may be a v-Src substrate. Several phosphorylation sites have been described in p130Cas upon Fibroblast Growth Factor stimulation, and phosphorylated tyrosine (Y249) might function as a binding site for the Crk-adaptor molecule. The J169-757.12.2 monoclonal antibody recognizes the phosphorylated Y249 of human p130Cas. The orthologous phosphorylation sites in mouse and rat p130Cas are Y253 and Y347, respectively.

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events, Platelet-derived growth Factor Receptor Signaling