CrkL anticorps (pTyr207) (PE)
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- Antigène Voir toutes CrkL (CRKL) Anticorps
- CrkL (CRKL) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL))
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Épitope
- pTyr207
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Reactivité
- Humain, Souris, Rat
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp CrkL est conjugé à/à la PE
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Application
- Intracellular Staining (ICS)
- Marque
- BD Phosflow™
- Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogène
- Phosphorylated Human, Mouse, and Rat CrkL Peptide
- Clone
- K30-391-50-80
- Isotype
- IgG2a kappa
- Top Product
- Discover our top product CRKL Anticorps primaire
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- Indications d'application
- This PE-conjugated antibody is suitable for intracellular staining of cell lines using BD Cytofix™ Fixation Buffer and BD Phosflow Perm Buffer III or IV. We have confirmed that the Alexa Fluor® 647 conjugate, Cat. No. 560790, is suitable for staining leukocytes from human peripheral blood and bone marrow.
- Commentaires
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BALB/c
- Volume d'échantillon
- 20 μL
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C
- Stockage commentaire
- The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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Crk family adaptors-signalling complex formation and biological roles." dans: Oncogene, Vol. 20, Issue 44, pp. 6348-71, (2001) (PubMed).
: "CrkL activates integrin-mediated hematopoietic cell adhesion through the guanine nucleotide exchange factor C3G." dans: Blood, Vol. 93, Issue 11, pp. 3713-22, (1999) (PubMed).
: "Structural requirements for function of the Crkl adapter protein in fibroblasts and hematopoietic cells." dans: Molecular and cellular biology, Vol. 18, Issue 9, pp. 5082-90, (1998) (PubMed).
: "
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Crk family adaptors-signalling complex formation and biological roles." dans: Oncogene, Vol. 20, Issue 44, pp. 6348-71, (2001) (PubMed).
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- Antigène
- CrkL (CRKL) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL))
- Autre désignation
- CrkL (CRKL Produits)
- Synonymes
- anticorps CRKL, anticorps wu:fa55g05, anticorps wu:fj60e01, anticorps zgc:63469, anticorps 1110025F07Rik, anticorps AA589403, anticorps AI325100, anticorps Crkol, anticorps CRK like proto-oncogene, adaptor protein, anticorps v-crk avian sarcoma virus CT10 oncogene homolog-like, anticorps CRKL, anticorps crkl, anticorps Crkl
- Sujet
- Crk-Like (CrkL) is an adaptor protein that is preferentially expressed in hematopoietic cells and is encoded by a gene that is homologous to the viral oncogene v-crk (chicken tumor virus no. 10 regulator of kinase). Its SH2 and SH3 domains bind to a variety of effector proteins, such as paxillin, p130Cas, c-Cbl, c-Abl, and C3G. These interactions are involved in the regulation of cellular migration, adhesion, and transformation. Tyrosine 207 (Y207) of CrkL is phosphorylated in hematopoietic cells that express the BCR-ABL fusion protein. This site may be a negative regulator of protein complex formation and biological activity. The K30-391.50.80 monoclonal antibody recognizes the phosphorylated Y207 of human CrkL.
- Pathways
- Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Signaling of Hepatocyte Growth Factor Receptor
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