Neutralization Activity: This antibody has been reported to be useful for the neutralization of recombinant mouse IL-2. Neutralization activity may be measured with a proliferation assay using 80 pg/mL recombinant mouse IL-2 (Cat. No. 550069) to stimulate CTLL-2 cells at 1x10^5 cells/mL as indicator cells (i.e. preincubation of the antibody with recombinant mouse IL-2 for 60 minutes). 50% Neutralization (ND50) at 5 - 30 ng/mL >95% Neutralization at 30 - 80 ng/mL
Purification
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Stérilité
0.2 μm filtered
niveau d'endotoxine
Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
IL2
Reactivité: Humain
WB
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Western Blot: The S4B6 antibody (purified format) has been reported to be useful for Western blotting. A concentration of 1-5 µg/mL (in conjunction with an AKP-conjugated goat anti-rat Ig secondary antibody) has been reported to enable detection of ≤ 100 ng/lane of recombinant mouse IL-2 (Cat. No. 550069) under reducing conditions.
Restrictions
For Research Use only
Format
Liquid
Concentration
1.0 mg/mL
Buffer
No azide/low endotoxin: Aqueous buffered solution containing protein stabilizer, no preservative, 0.2μm sterile filtered.
Agent conservateur
Azide free
Stock
4 °C
Stockage commentaire
Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Svetić, Jian, Lu, Finkelman, Gause: "Brucella abortus induces a novel cytokine gene expression pattern characterized by elevated IL-10 and IFN-gamma in CD4+ T cells." dans: International immunology, Vol. 5, Issue 8, pp. 877-83, (1993) (PubMed).
Kubo, Cinader: "Polymorphism of age-related changes in interleukin (IL) production: differential changes of T helper subpopulations, synthesizing IL 2, IL 3 and IL 4." dans: European journal of immunology, Vol. 20, Issue 6, pp. 1289-96, (1990) (PubMed).
Gillis, Ferm, Ou, Smith: "T cell growth factor: parameters of production and a quantitative microassay for activity." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 120, Issue 6, pp. 2027-32, (1978) (PubMed).
The S4B6 antibody reacts with mouse interleukin-2 (IL-2). The immunogen used to generate the S4B6 hyridoma was a T cell clone supernatant. This is a neutralizing antibody.