FAS anticorps

N° du produit ABIN1177304

L’anticorps Hamster arménien Monoclonal anti-FAS a été validé pour FACS, IP et CyTox. Il convient pour détecter FAS dans des échantillons de Souris. Il y a 9+ publications disponibles.

Aperçu rapide pour FAS anticorps (ABIN1177304)

Antigène: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Reactivité: Souris

Hôte: Hamster arménien

Clonalité: Monoclonal

Conjugué: Cet anticorp FAS est non-conjugé

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Cytotoxicity Test (CyTox)

Clone: Jo2

Détail du produit anti-FAS anticorps

Marque: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogène: WR19L mouse lymphoma cells transformed with recombinant mouse Fas

Isotype: IgG2 lambda

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Agent conservateur: Azide free

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Références pour anticorps anti-FAS (ABIN1177304)

Enari, Hug, Nagata: "Involvement of an ICE-like protease in Fas-mediated apoptosis." dans: Nature, Vol. 375, Issue 6526, pp. 78-81, (1995) (PubMed).

Nagata: "Apoptosis regulated by a death factor and its receptor: Fas ligand and Fas." dans: Philosophical transactions of the Royal Society of London. Series B, Biological sciences, Vol. 345, Issue 1313, pp. 281-7, (1995) (PubMed).

Nagata: "Fas and Fas ligand: a death factor and its receptor." dans: Advances in immunology, Vol. 57, pp. 129-44, (1995) (PubMed).

Ni, Tomita, Matsuda, Ichihara, Ishimura, Ogasawara, Nagata: "Fas-mediated apoptosis in primary cultured mouse hepatocytes." dans: Experimental cell research, Vol. 215, Issue 2, pp. 332-7, (1995) (PubMed).

Ogasawara, Suda, Nagata: "Selective apoptosis of CD4+CD8+ thymocytes by the anti-Fas antibody." dans: The Journal of experimental medicine, Vol. 181, Issue 2, pp. 485-91, (1995) (PubMed).

Yang, Merćep, Ware, Ashwell: "Fas and activation-induced Fas ligand mediate apoptosis of T cell hybridomas: inhibition of Fas ligand expression by retinoic acid and glucocorticoids." dans: The Journal of experimental medicine, Vol. 181, Issue 5, pp. 1673-82, (1995) (PubMed).

Hiromatsu, Aoki, Makino, Matsumoto, Mizuochi, Gotoh, Nomoto, Ogasawara, Nagata, Yoshikai: "Increased Fas antigen expression in murine retrovirus-induced immunodeficiency syndrome, MAIDS." dans: European journal of immunology, Vol. 24, Issue 10, pp. 2446-51, (1994) (PubMed).

Kägi, Vignaux, Ledermann, Bürki, Depraetere, Nagata, Hengartner, Golstein: "Fas and perforin pathways as major mechanisms of T cell-mediated cytotoxicity." dans: Science (New York, N.Y.), Vol. 265, Issue 5171, pp. 528-30, (1994) (PubMed).

Ogasawara, Watanabe-Fukunaga, Adachi, Matsuzawa, Kasugai, Kitamura, Itoh, Suda, Nagata: "Lethal effect of the anti-Fas antibody in mice." dans: Nature, Vol. 364, Issue 6440, pp. 806-9, (1993) (PubMed).

Détails sur FAS

Antigène: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Autre désignation: CD95

Sujet: Fas antigen, CD95, is a 45 kDa cell-surface protein which can mediate apoptosis. It belongs to the TNF (tumor necrosis factor)/NGF receptor family. Expression of Fas has been described in the thymus, liver, heart, lung and ovary. Fas plays an important role in the apoptotic process that takes place during development. Monoclonal antibodies recognizing Fas such as Jo2 have cytolytic activity on cells expressing Fas. The cell death stimulated by Fas antibodies is characteristic of apoptosis and suggests that the lethal effects are a result of interaction of antibody with a functional Fas antigen as opposed to complement-mediated lysis. The Jo2 antibody recognizes mouse Fas. The Jo2 antibody shows cytolytic activity against cell lines expressing mouse Fas by inducing apoptosis. Intraperitoneal injections of Jo2 mAb have been shown to kill mice and induce apoptotic hepatocyte death. Jo2 mAb has been reported to immunoprecipitate mouse Fas as a 45 kDa band from W4 cells. W4 cells are WR19L mouse lymphoma cells transformed with mouse Fas. The difference between the observed MW of Fas and that deduced from its amino acid sequence (Mr 34,971) may be due to glycosylation.
Synonyms: Fas/APO-1

Poids moléculaire: 45 kDa

Pathways: Signalisation p53, Apoptose, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity