CXCR4 anticorps

N° du produit ABIN1177332

Cet anticorps anti-CXCR4 est un anticorps Souris Monoclonal détectant CXCR4 dans FACS. Adapté pour Humain. Ce Primary Antibody a été cité dans 3+ publications.

Aperçu rapide pour CXCR4 anticorps (ABIN1177332)

Antigène: CXCR4 (Chemokine (C-X-C Motif) Receptor 4 (CXCR4))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CXCR4 est non-conjugé

Application: Flow Cytometry (FACS)

Clone: 12G5

Détail du produit anti-CXCR4 anticorps

Marque: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Isotype: IgG2a kappa

Information d'application

Indications d'application: Flow cytometry: Chemokine receptors are known to internalize during manipulation resulting in low frequency expression. Investigators are advised to perform immunophenotyping studies of chemokine receptors on freshly collected samples (<24 Hrs). Incubation with the antibody should be done at 4°C in the dark. Cellular manipulation, such as Ficoll separation, freezing, or exposure to cold temperatures prior to staining should be minimized and have been shown to cause a decrease in staining intensity and/or inconsistent results. Investigators should note that alternative staining procedures may be neccessary. A multiple-step staining procedure is strongly recommended, in some instances, to amplify immunofluorescent signals for the flow cytometric analysis of human CXCR4 expression. Investigators may find the Purified NA/LE Mouse Anti-Human CD184 antibody to be useful in conjunction with appropriate secondary and tertiary reagents for detecting low frequency expression, such as with Biotin Goat Anti-Mouse Ig (MN 553999) and PE Streptavidin (MN 554061).

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Agent conservateur: Azide free

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Références pour anticorps anti-CXCR4 (ABIN1177332)

Simmons, Wilkinson, Reeves, Dittmar, Beddows, Weber, Carnegie, Desselberger, Gray, Weiss, Clapham: "Primary, syncytium-inducing human immunodeficiency virus type 1 isolates are dual-tropic and most can use either Lestr or CCR5 as coreceptors for virus entry." dans: Journal of virology, Vol. 70, Issue 12, pp. 8355-60, (1997) (PubMed).

Endres, Clapham, Marsh, Ahuja, Turner, McKnight, Thomas, Stoebenau-Haggarty, Choe, Vance, Wells, Power, Sutterwala, Doms, Landau, Hoxie: "CD4-independent infection by HIV-2 is mediated by fusin/CXCR4." dans: Cell, Vol. 87, Issue 4, pp. 745-56, (1997) (PubMed).

Feng, Broder, Kennedy, Berger: "HIV-1 entry cofactor: functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor." dans: Science (New York, N.Y.), Vol. 272, Issue 5263, pp. 872-7, (1996) (PubMed).

Détails sur CXCR4

Antigène: CXCR4 (Chemokine (C-X-C Motif) Receptor 4 (CXCR4))

Autre désignation: CD184

Sujet: Reacts with fusin (CXCR4), a seven-transmembrane domain, G-protein-linked glycoprotein. Fusin is expressed on a wide variety of CD4+ and CD4- cells including those from spleen, brain and lung. It has been shown to function as a cofactor for infection with T-cell tropic strains of HIV-1 and also as a receptor for CD4-independent infection. 12G5 antibody has been reported to block CD4-independent infection by HIV-2 and CD4-dependent infection by some T-cell tropic isolates of HIV-1.
Synonyms: CXCR4 (Fusin)

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events