SKP1 anticorps (C-Term)

N° du produit ABIN117959

Il existe 1 publication pour ce produit. L’anticorps anti-SKP1 Polyclonal Lapin est utilisé pour la détection de SKP1 dans des échantillons de Humain. Il a été validé pour WB, IP et EIA.

Aperçu rapide pour SKP1 anticorps (C-Term) (ABIN117959)

Antigène: SKP1 (S-Phase Kinase-Associated Protein 1 (SKP1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SKP1 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Détail du produit anti-SKP1 anticorps

Épitope: AA 152-163, C-Term

Séquence: Human SKP1 (163 aa 18.5 kDa) 1 mpsiklqssd geifevdvei akqsvtiktm ledlgmddeg dddpvplpnv naailkkviq 61 wcthhkddpp ppeddenkek rtddipvwdq eflkvdqgtl felilaanyl dikglldvtc 121 ktvanmikgk tpeeirktfn ikndfteeee aqvrkenqwc eek

Specificité: This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human SKP1. Cross reactivity is expected against mouse SKP1 based on a high degree of sequence homology. Cross reactivity with other SKP1 proteins or SKP1 from other sources is not known.

Purification: Delipidation and defibrination.

Immunogène: Prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 152-163 of Human SKP1 (C-terminus) coupled to KLH.

Information d'application

Indications d'application: ELISA (1/2,000-1/10,000). This antibody reacts with human SKP1 by Western blot(1/500-1/1,000) and Immunoprecipitation. The antibody Immunoprecipitates in vitrotranslated protein and protein from cell lysates (using HeLa, NIH-3T3, and others). Coimmunoprecipitation of cyclin A and Cuol1 may occur. An 18.5 kDa band correspondingto human SKP1 is detected. Most cell lines expressing SKP1 can be used as a positivecontrol.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 85.0 mg/mL (by Refractometry)

Stock: 4 °C

Stockage commentaire: Store undiluted at 2-8 °C.

Références pour anticorps anti-SKP1 (ABIN117959)

Wang, He, Wang, Wang, Zhai, Zhou, Zhang, Zhai, Feng, Han: "[Impact of Donor KIR2DS5 Genotype on Outcome Following Haploidentical Hematopoietic Stem Cell Transplantation.]" dans: Zhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology, Vol. 16, Issue 1, pp. 111-5, (2008) (PubMed).

Détails sur SKP1

Antigène: SKP1 (S-Phase Kinase-Associated Protein 1 (SKP1))

Autre désignation: SKP1 / OCP2

Sujet: SKP1 is also known as S-phase kinase-associated protein 1A, Cyclin A/CDK2-associated protein p19, p19A, p19skp1, RNA polymerase II elongation factor-like protein, Organ of Corti protein 2, OCP-II protein, OCP-2, Transcription elongation factor B, and SIII. SKP1 is an essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, SKP1 serves as an adapter that links the F-box protein to CUL1. SKP1 interacts directly with CUL1 and F-box proteins, such as BTRC and SKP2, in the SCF complex. SKP1 also interacts with FBX29 and the cyclin A/CDK2 complex. SKP1 is part of a SCF-like complex consisting of CUL7, RBX1, SKP1 and FBXW8 and is also a component of a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1A, APC and TBL1X.Synonyms: Cyclin A/CDK2-associated protein p19, EMC19, OCP-2, OCP-II, Organ of Corti protein 2, RNA polymerase II elongation factor-like protein, S-phase kinase-associated protein 1, SKP1A, TCEB1L, Transcription elongation factor B, p19A, p19skp1

ID gène: 6500

NCBI Accession: NP_008861

UniProt: P63208

Pathways: Cycle Cellulaire, Signalisation Hedgehog, Mitotic G1-G1/S Phases