CD3 anticorps (APC)

N° du produit ABIN118100

L’anticorps Rat Monoclonal anti-CD3 a été validé pour FACS. Il convient pour détecter CD3 dans des échantillons de Souris.

Aperçu rapide pour CD3 anticorps (APC) (ABIN118100)

Antigène: CD3

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CD3 est conjugé à/à la APC

Application: Flow Cytometry (FACS)

Clone: KT3

Détail du produit anti-CD3 anticorps (APC)

Specificité: This antibody recognises the CD3 antigen which is expressed by mature T cells.

Purification: Affinity chromatography on Protein A

Immunogène: Spleen cells from immunised SD rats were fused with cells of the NSO mouse myeloma cell line.

Isotype: IgG2a

Information d'application

Indications d'application: Flow Cytometry: For optimal staining incubations should be performed at roomtemperature, The Fc region of monoclonal antibodies may bind non-specifically to cellsexpressing low affinity Fc receptors.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 1.0 mL distilled water

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin

Agent conservateur: Sodium azide

Précaution d'utilisation: Use of Sodium Szide as a preservative will substantially inhibit the enzyme activity of Horseradish Peroxidase.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur CD3

Antigène: CD3

Autre désignation: CD3

Sujet: T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4

ID gène: 12501

NCBI Accession: NP_031674

UniProt: P22646

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway