CD4 anticorps

N° du produit ABIN118115

L’anticorps Rat Monoclonal anti-CD4 a été validé pour FACS et IHC (fro). Il convient pour détecter CD4 dans des échantillons de Souris.

Aperçu rapide pour CD4 anticorps (ABIN118115)

Antigène: CD4

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CD4 est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: YTS191-1

Détail du produit anti-CD4 anticorps

Specificité: This antibody recognises the CD4 cell surface antigen, expressed by a subset of T-lymphocytes. This clone exhibits depleting activity when used in vivo (Azide Free CD4 Antibody Cat.-No SM005LE is recommended for this purpose).

Purification: Affinity Chromatography on Protein G

Isotype: IgG2b

Information d'application

Indications d'application: Flow Cytometry: Use 10 μL of 1/50-1/200 diluted antibody to label 10⁶ cells.
Immunohistochemistry on Frozen Sections: The epitope recognised by this antibody is reported to be sensitive to Formaldehyde Fixation and tissue processing. The use of Acetone fixation for frozen sections is recommended.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur CD4

Antigène: CD4

Autre désignation: CD4

Sujet: CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

ID gène: 12504

NCBI Accession: NP_038516

UniProt: P06332

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events