P-Selectin anticorps

N° du produit ABIN118767

Cet anticorps anti-P-Selectin est un anticorps Souris Monoclonal détectant P-Selectin dans WB, FACS, IHC (fro) et IHC (p). Adapté pour Humain et Singe.

Aperçu rapide pour P-Selectin anticorps (ABIN118767)

Antigène: P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))

Reactivité: Humain, Singe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp P-Selectin est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: AK-6

Détail du produit anti-P-Selectin anticorps

Specificité: This antibody recognizes CD62P. It reacts with a 140 kDa antigen expressed on activated platelets and also on endothelial cells both of blood and lymph vessels.

Purification: Affinity Chromatography on Protein G

Immunogène: Human platelet membrane glycoproteins.

Isotype: IgG1

Information d'application

Indications d'application: Flow Cytometry: Use 10 μL of 1/10-1/100 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen Sections (1/1000-1/5000) and Paraffin Embedded Sections (1/500-1/2000): This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections but staining may be enhanced using Sodium Citrate buffer pH 6.0. Recommended Positive Control Tissue: Tonsil. Western blotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing. Dilute only prior to immediate use.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the product undiluted at 2-8 °C for up to one month. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below.

Détails sur P-Selectin

Antigène: P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))

Autre désignation: CD62P / P-Selectin

Sujet: CD62P (P-Selectin) is an adhesion glycoprotein that is expressed on platelets and endothelial cells upon their activation. Interaction between CD62P and its mucin-like ligand PSGL-1 (P-selectin glycoprotein ligand-1) expressed on the microvilli of most leukocytes supports leukocyte rolling along postkapillary venules at the earliest time of inflammation. Both CD62P and PSGL-1 are extended glycoproteins that form homodimers. CD62P dimerization is probably mediated through interactions of the transmembrane domains and stabilizes leukocyte tethering and rolling, probably by increasing rebinding within a bond cluster.Synonyms: CD62 antigen-like family member P, GMP-140, GMRP, GRMP, Granule membrane protein 140, LECAM3, Leukocyte-endothelial cell adhesion molecule 3, PADGEM, SELP

ID gène: 6403

NCBI Accession: NP_002996

UniProt: P16109