C5AR1 anticorps (N-Term)

N° du produit ABIN118843

Cet anticorps Souris Monoclonal détecte spécifiquement C5AR1 dans WB, FACS et IHC (p). Il présente une réactivité avec des échantillons de Humain, Lapin et Boeuf (Vache).

Aperçu rapide pour C5AR1 anticorps (N-Term) (ABIN118843)

Antigène: C5AR1 (Complement Component 5a Receptor 1 (C5AR1))

Reactivité: Humain, Lapin, Boeuf (Vache)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp C5AR1 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: S5-1

Détail du produit anti-C5AR1 anticorps

Épitope: AA 1-31, N-Term

Specificité: This antibody recognises the C5a receptor (C5aR) CD88. Clone S5/1 was raised against a synthetic peptide comprising the N-terminal extracellular domain of the C5aR (met1-Asn31) and has recently been shown to recognise the heptameric peptide (D15DKDTLD21). Clone S5/1 has been shown to inhibit the binding of C5a to its receptor. We recommend the use of SM1182A for functional studies.

Réactivité croisée (Details): Species reactivity (expected):Bovine and Rabbit.
Species reactivity (tested):Human

Purification: Ammonium sulphate precipitation

Immunogène: C5aR - peptide: Met1 - Asn31. Spleen cells from immunised BALB/c mice were fused with cells of the X63-Ag8 myeloma cell line.

Isotype: IgG2a

Information d'application

Indications d'application: Flow Cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 5 x 10e5 cells in 100 μL. Immunohistochemistry on paraffin sections. Western Blot. Functional Assays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur C5AR1

Antigène: C5AR1 (Complement Component 5a Receptor 1 (C5AR1))

Autre désignation: CD88 / C5R1

Sujet: CD88 is predominantly expressed on cells of the myeloid lineage.Synonyms: C5AR1, C5a anaphylatoxin chemotactic receptor, C5a-R, C5aR, Complement Component 5a Receptor 1

ID gène: 728

NCBI Accession: NP_001727

UniProt: P21730

Pathways: Système du Complément