HLA-ABC anticorps

N° du produit ABIN118899

Cet anticorps anti-HLA-ABC est un anticorps Souris Monoclonal détectant HLA-ABC dans FACS, IP, IHC (fro), IF, Func, EIA et DNA Mic. Adapté pour Humain, Boeuf (Vache), Babouin, Macaque, Cat, Singe, Primate et Chimpanzé. Ce Primary Antibody a été cité dans 4+ publications.

Aperçu rapide pour HLA-ABC anticorps (ABIN118899)

Antigène: HLA-ABC

Reactivité: Humain, Boeuf (Vache), Babouin, Macaque, Cat, Singe, Primate, Chimpanzé

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp HLA-ABC est non-conjugé

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Functional Studies (Func), Enzyme Immunoassay (EIA), DNA Microscopy (DNA Mic)

Clone: W6-32

Détail du produit anti-HLA-ABC anticorps

Purification: Affinity Chromatography on Protein G

niveau d'endotoxine: Less than 0.01 EU/μg

Immunogène: Purified Human tonsil lymphocyte membranes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1/1-Ag4.1 myeloma cell line

Isotype: IgG2a

Information d'application

Indications d'application: This product is routinely tested in flow cytometry on human peripheral blood lymphocytes: Use 10 μL of 1/50 - 1/100 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections (see Ref 6, Tonsil is useful as positive control). Not suitable for Immunohistochemistry on paraffin sections. Immunoprecipitation (Ref 1). ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS without preservatives or stabilizers.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Références pour anticorps anti-HLA-ABC (ABIN118899)

Giersch, Hermanussen, Volz, Volmari, Allweiss, Sureau, Casey, Huang, Fischer, Lütgehetmann, Dandri: "Strong Replication Interference Between Hepatitis Delta Viruses in Human Liver Chimeric Mice." dans: Frontiers in microbiology, Vol. 12, pp. 671466, (2021) (PubMed).

Stern, Goldman, Hatasaka, Mackenzie, Surrey, Racowsky,: "Optimizing the number of cleavage stage embryos to transfer on day 3 in women 38 years of age and older: a Society for Assisted Reproductive Technology database study." dans: Fertility and sterility, (2008) (PubMed).

Mayr, Bund, Schlee, Moosmann, Kofler, Hallek, Wendtner: "Fibromodulin as a novel tumor-associated antigen (TAA) in chronic lymphocytic leukemia (CLL), which allows expansion of specific CD8+ autologous T lymphocytes." dans: Blood, Vol. 105, Issue 4, pp. 1566-73, (2005) (PubMed).

Mayr, Kofler, Büning, Bund, Hallek, Wendtner: "Transduction of CLL cells by CD40 ligand enhances an antigen-specific immune recognition by autologous T cells." dans: Blood, Vol. 106, Issue 9, pp. 3223-6, (2005) (PubMed).

Détails sur HLA-ABC

Antigène: HLA-ABC

Autre désignation: HLA Class I ABC

Sujet: Synonyms: HLA Class 2 ABC, Human Leukocyte antigen class I ABC, MHC class I ABC, Major Histocompatibility complex class I

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints