CD3 anticorps (FITC)

N° du produit ABIN119605

Cet anticorps Souris Monoclonal détecte spécifiquement CD3 dans FACS. Il présente une réactivité envers Macaque rhésus.

Aperçu rapide pour CD3 anticorps (FITC) (ABIN119605)

Antigène: CD3

Reactivité: Macaque rhésus

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD3 est conjugé à/à la FITC

Application: Flow Cytometry (FACS)

Clone: FN-18

Détail du produit anti-CD3 anticorps (FITC)

Purification: Affinity Chromatography on Protein A

Immunogène: Rhesus monkey peripheral blood leucocytes. Spleen cells from immunised mice were fused with cells of the mouse SP2/0 Ag14 myeloma cell line.

Isotype: IgG1

Information d'application

Indications d'application: Flow cytometry: 1/10, Use 50 μL of the suggested working dilution to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 0.5 mL distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. We recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09 % Sodium Azide and 1 % BSA is recommended.

Buffer: PBS containing 1 % BSA and 125 mM Trehalose

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur CD3

Antigène: CD3

Autre désignation: CD3

Sujet: T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4

ID gène: 9541

UniProt: Q95LI5

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway